{"title":"高效液相色谱法测定大鼠血浆中格列酮的含量","authors":"S. Sridevi, P. Diwan","doi":"10.1211/146080800128736097","DOIUrl":null,"url":null,"abstract":"A HPLC method has been developed for determination of gliquidone in rat plasma. The assay involves combined extraction and precipitation with 1:1 methanol-acetonitrile, and separation of the analyte on a Shimpack ODS (C18) column with 75:25 (v/v) acetonitrile-0.1 M acetic acid as mobile phase. Detection at 229 nm was by photodiode-array detection. The assay was validated in accordance with international requirements and found to be specific, accurate and precise with a linearity range from 50 ng mL−1 to 10μg mL−1. The method was suitable for conducting pharmacokinetic studies in rats.","PeriodicalId":19946,"journal":{"name":"Pharmacy and Pharmacology Communications","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"Validated HPLC Method for the Determination of Gliquidone in Rat Plasma\",\"authors\":\"S. Sridevi, P. Diwan\",\"doi\":\"10.1211/146080800128736097\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A HPLC method has been developed for determination of gliquidone in rat plasma. The assay involves combined extraction and precipitation with 1:1 methanol-acetonitrile, and separation of the analyte on a Shimpack ODS (C18) column with 75:25 (v/v) acetonitrile-0.1 M acetic acid as mobile phase. Detection at 229 nm was by photodiode-array detection. The assay was validated in accordance with international requirements and found to be specific, accurate and precise with a linearity range from 50 ng mL−1 to 10μg mL−1. The method was suitable for conducting pharmacokinetic studies in rats.\",\"PeriodicalId\":19946,\"journal\":{\"name\":\"Pharmacy and Pharmacology Communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacy and Pharmacology Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1211/146080800128736097\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacy and Pharmacology Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1211/146080800128736097","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Validated HPLC Method for the Determination of Gliquidone in Rat Plasma
A HPLC method has been developed for determination of gliquidone in rat plasma. The assay involves combined extraction and precipitation with 1:1 methanol-acetonitrile, and separation of the analyte on a Shimpack ODS (C18) column with 75:25 (v/v) acetonitrile-0.1 M acetic acid as mobile phase. Detection at 229 nm was by photodiode-array detection. The assay was validated in accordance with international requirements and found to be specific, accurate and precise with a linearity range from 50 ng mL−1 to 10μg mL−1. The method was suitable for conducting pharmacokinetic studies in rats.
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