蛋清水解物抗氧化活性评价及其在肉片颜色稳定性中的应用

Yu Wang, Jufang Shen, H. Hatta
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引用次数: 2

摘要

在这项研究中,我们评估了四种不同大小分布的蛋清(EW)水解物的抗氧化效果,并评估了它们作为天然抗氧化剂用于肉类保鲜的潜力。测定了EW水解产物的水溶性和氧自由基吸收能力(ORAC)。与天然蛋清相比,水解使EW水解产物的ORAC值增加。在此基础上,研究了EW水解物对猪肉片颜色稳定性的影响。将猪肉片分别浸泡在四种蛋白质浓度为10% (w/w)的EW水解液中。4℃保存24 h后,用比色仪客观测定猪肉片的颜色,并测定浸泡前后的甲氧肌红蛋白含量。小尺寸EW水解液S具有良好的ORAC值(389µmol TE/g)和良好的水溶性,抑制了处理过的猪肉片的颜色变化(ΔE = 2.8),并且在处理过的猪肉中发现了最低的肌红蛋白比例,并且表明EW水解液在改善保存期间的颜色稳定性方面普遍优于对照(仅用水)。这些结果表明,EW水解物是一种很有前途的生物抗氧化剂来源,可以用作肉制品的防腐剂。
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Evaluation of Antioxidant Activity of Egg White Hydrolysates and Their Application on Color Stability of Pork Meat Slices
In this study, we assessed the antioxidant efficacy of four different size distributions of Egg White (EW) hydrolysates and evaluated their potential as natural antioxidants for meat preservation. Water solubility and Oxygen Radical Absorbance Capacity (ORAC) of EW hydrolysates were measured. Hydrolysis contributed to the increase of ORAC values of EW hydrolysates in comparison with native egg white. Based on this result, we investigated the effects of EW hydrolysates on color stability of pork slices. Pork slices were individually soaked in four EW hydrolysates solutions at 10% (w/w) protein concentration. After storage at 4°C for 24 h, objective measurements of the color of pork slices were performed using a colorimeter, the content of metmyoblobin before and after soak was also determined. Small size EW hydrolysate S showed a good ORAC value (389 µmol TE/g) and an excellent water solubility, inhibited color change (ΔE = 2.8) in treated pork slices, the lowest metmyoglobin proportion was also found in the meat treated by this small size EW hydrolysate S. Moreover, it was demonstrated that EW hydrolysates were generally better than the Control (only with water) in improving color stability during storage time. These results indicate that EW hydrolysates are promising sources of biological antioxidants and can potentially be used as preservatives in meat products.
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