Effect of Injectable-Platelet Rich Fibrin on marginal adaptation of Bioactive Materials Used as Direct Pulp Capping; An Experimental Animal Study

Introduction: Vital pulp therapy has been known as one of the treatment options to preserve pulp vitality after being exposed by trauma or caries. Aim: This experiment explored the effect of injectable-Platelet Rich Fibrin on marginal adaptation of two pulp capping agents (Mineral Trioxide Aggregate and Bioactive Bone Graft). Materials and methods: A total of 64 teeth were used out of 8 healthy male beagle dogs. The teeth were randomly assigned into four groups, they were exposed and capped with different capping agents. Group A; capped with Mineral Trioxide Aggregate (MTA), Group B; capped with MTA+ i-PRF, Group C; capped with Bioactive Bone Graft (BBG), Group D; capped with BBG+i-PRF. Finally the access cavity was restored with Intermediate Restorative Material (IRM). At each predetermined interval, the dogs were sacrificed (1 month, and 3 months). The samples were then prepared for electron microscopic scanning evaluation. To compare between the gap percentage of four groups at each interval, Kruskal-wallis test; was used. Mann-Whitney U test; was used to pair-wise comparison when Kruskal-wallis test is significant. Bonferroni’s correction was utilized for the pair-wise comparisons. Statistical significance was considered at P < .05. Results: The data revealed that after one and three months the best values were recorded in groups B (MTA+ i-PRF) and D (BBG+ i-PRF), in relation to the lowest gap area between the capping materials and dentin, followed by group C (BBG), with the least value recorded in group A (MTA). Conclusion: the findings from the current study suggested that i-PRF provided a better marginal adaptation of either MTA or BBG to the pulp and dentin, which improved with time from one month to three months.