{"title":"用高灵敏度和特异性的聚合酶链反应方法检测人类免疫缺陷病毒1型","authors":"FY-T Tung","doi":"10.1016/0888-0786(94)90036-1","DOIUrl":null,"url":null,"abstract":"<div><p>A sensitive and specific polymerase chain reaction (PCR)-based assay was developed for detection of a single copy of human immunodeficiency virus type 1 (HIV-1) sequence. The different methodologies for preparation of clinical DNA samples were evaluated. The DNA extracted by the Ficoll-Histopaque method gave the best quality for PCR. DNA samples equivalent to 18 μl or less of blood from HIV-1 seropositive individuals were positive by this assay. This procedure should be suitable for early diagnosis of HIV-1 infection in many clinical situations.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"6 4","pages":"Pages 218-220"},"PeriodicalIF":0.0000,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0888-0786(94)90036-1","citationCount":"2","resultStr":"{\"title\":\"Detection of human immunodeficiency virus type 1 by a highly sensitive and specific polymerase chain reaction method\",\"authors\":\"FY-T Tung\",\"doi\":\"10.1016/0888-0786(94)90036-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A sensitive and specific polymerase chain reaction (PCR)-based assay was developed for detection of a single copy of human immunodeficiency virus type 1 (HIV-1) sequence. The different methodologies for preparation of clinical DNA samples were evaluated. The DNA extracted by the Ficoll-Histopaque method gave the best quality for PCR. DNA samples equivalent to 18 μl or less of blood from HIV-1 seropositive individuals were positive by this assay. This procedure should be suitable for early diagnosis of HIV-1 infection in many clinical situations.</p></div>\",\"PeriodicalId\":101161,\"journal\":{\"name\":\"Serodiagnosis and Immunotherapy in Infectious Disease\",\"volume\":\"6 4\",\"pages\":\"Pages 218-220\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0888-0786(94)90036-1\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Serodiagnosis and Immunotherapy in Infectious Disease\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0888078694900361\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Serodiagnosis and Immunotherapy in Infectious Disease","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0888078694900361","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Detection of human immunodeficiency virus type 1 by a highly sensitive and specific polymerase chain reaction method
A sensitive and specific polymerase chain reaction (PCR)-based assay was developed for detection of a single copy of human immunodeficiency virus type 1 (HIV-1) sequence. The different methodologies for preparation of clinical DNA samples were evaluated. The DNA extracted by the Ficoll-Histopaque method gave the best quality for PCR. DNA samples equivalent to 18 μl or less of blood from HIV-1 seropositive individuals were positive by this assay. This procedure should be suitable for early diagnosis of HIV-1 infection in many clinical situations.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
