{"title":"纳秒脉冲电场对肿瘤细胞及抗癌药物联合的影响","authors":"Soichiro Enomoto, Daisuke Konishi, Yoshihiro Uto, Naoyuki Shimomura","doi":"10.1002/eej.23376","DOIUrl":null,"url":null,"abstract":"<p>With the goal of establishing a new cancer treatment method using nanosecond pulsed electric fields (nsPEFs), we investigated the effect of applying nsPEFs and anticancer drugs (adriamycin, ADM) to cancer cells. The surviving fraction of cancer cells was significantly reduced by nsPEFs application compared to the control. Compared to nsPEFs alone and ADM administration alone, a significant decrease of the surviving fraction was observed in combination of nsPEFs application and ADM administration. In addition, multiplying value of surviving fractions of nsPEFs application and ADM administration was calculated as a pseudo-combination effect. The surviving fractions of the experimental combinational results of nsPEFs and ADM were lower than the pseudo-combination results. Therefore, the synergistic effect of combination of nsPEFs and ADM was confirmed. Furthermore, the surviving fraction of ADM administration after nsPEFs application was significantly lower than that of ADM administration on 24 h before nsPEFs application. ADM action on cells would be effective owing to nanopores were formed by applying nsPEFs. It was confirmed that ADM and nsPEF induced apoptosis of cells from apoptosis and necrosis test using FACS.</p>","PeriodicalId":50550,"journal":{"name":"Electrical Engineering in Japan","volume":"215 2","pages":""},"PeriodicalIF":0.4000,"publicationDate":"2022-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Effects of nanosecond pulsed electric fields application on cancer cell and combination of anticancer drug\",\"authors\":\"Soichiro Enomoto, Daisuke Konishi, Yoshihiro Uto, Naoyuki Shimomura\",\"doi\":\"10.1002/eej.23376\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>With the goal of establishing a new cancer treatment method using nanosecond pulsed electric fields (nsPEFs), we investigated the effect of applying nsPEFs and anticancer drugs (adriamycin, ADM) to cancer cells. The surviving fraction of cancer cells was significantly reduced by nsPEFs application compared to the control. Compared to nsPEFs alone and ADM administration alone, a significant decrease of the surviving fraction was observed in combination of nsPEFs application and ADM administration. In addition, multiplying value of surviving fractions of nsPEFs application and ADM administration was calculated as a pseudo-combination effect. The surviving fractions of the experimental combinational results of nsPEFs and ADM were lower than the pseudo-combination results. Therefore, the synergistic effect of combination of nsPEFs and ADM was confirmed. Furthermore, the surviving fraction of ADM administration after nsPEFs application was significantly lower than that of ADM administration on 24 h before nsPEFs application. ADM action on cells would be effective owing to nanopores were formed by applying nsPEFs. It was confirmed that ADM and nsPEF induced apoptosis of cells from apoptosis and necrosis test using FACS.</p>\",\"PeriodicalId\":50550,\"journal\":{\"name\":\"Electrical Engineering in Japan\",\"volume\":\"215 2\",\"pages\":\"\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2022-03-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electrical Engineering in Japan\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/eej.23376\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electrical Engineering in Japan","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/eej.23376","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 3
摘要
目标是建立一个新的癌症治疗方法使用纳秒脉冲电场(nsPEFs),我们调查的影响,应用nsPEFs和抗癌药物(阿霉素(ADM)癌细胞。与对照组相比，应用nsPEFs显著降低了癌细胞的存活比例。与单独使用nsPEFs和单独使用ADM相比，nsPEFs和ADM联合使用显著降低了存活分数。此外，计算nsPEFs施用与ADM施用的存活分数的乘积值，作为伪组合效应。幸存的分数nsPEFs和ADM的实验组合结果低于pseudo-combination结果。因此，nsPEFs与ADM联合使用的协同效应得到了证实。此外，施用nsPEFs后ADM的存活率显著低于施用前24 h ADM的存活率。由于施加nspf形成纳米孔，因此ADM对细胞的作用有效。流式细胞术证实ADM和nsPEF均能诱导细胞凋亡。
Effects of nanosecond pulsed electric fields application on cancer cell and combination of anticancer drug
With the goal of establishing a new cancer treatment method using nanosecond pulsed electric fields (nsPEFs), we investigated the effect of applying nsPEFs and anticancer drugs (adriamycin, ADM) to cancer cells. The surviving fraction of cancer cells was significantly reduced by nsPEFs application compared to the control. Compared to nsPEFs alone and ADM administration alone, a significant decrease of the surviving fraction was observed in combination of nsPEFs application and ADM administration. In addition, multiplying value of surviving fractions of nsPEFs application and ADM administration was calculated as a pseudo-combination effect. The surviving fractions of the experimental combinational results of nsPEFs and ADM were lower than the pseudo-combination results. Therefore, the synergistic effect of combination of nsPEFs and ADM was confirmed. Furthermore, the surviving fraction of ADM administration after nsPEFs application was significantly lower than that of ADM administration on 24 h before nsPEFs application. ADM action on cells would be effective owing to nanopores were formed by applying nsPEFs. It was confirmed that ADM and nsPEF induced apoptosis of cells from apoptosis and necrosis test using FACS.
期刊介绍：
Electrical Engineering in Japan (EEJ) is an official journal of the Institute of Electrical Engineers of Japan (IEEJ). This authoritative journal is a translation of the Transactions of the Institute of Electrical Engineers of Japan. It publishes 16 issues a year on original research findings in Electrical Engineering with special focus on the science, technology and applications of electric power, such as power generation, transmission and conversion, electric railways (including magnetic levitation devices), motors, switching, power economics.
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