辐照牛肝线粒体DNA的变化:一种检测方法

E. Marchioni , M. Tousch , V. Zumsteeg , F. Kuntz , C. Hasselmann
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引用次数: 3

摘要

研究DNA的放射性降解是研究辐照食品鉴别方法的众多现象之一。牛肝线粒体DNA在辐射下的特定行为使检测产品是否受到辐射成为可能。最终可以开发出一种鉴定方法。线粒体超螺旋DNA片段的鉴定构成了牛肝辐照的明确检测试验。总共有120个不同的样品被以5种不同的剂量照射。检测的剂量限低于2kgy。没有观察到由于储存条件的影响。
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Alterations of mitochondrial DNA: A method for the detection of irradiated beef liver

The study of the radio degradation of DNA is one of a number of phenomena being investigated to develop methods for identifying irradiated foods. The specific behaviour under radiation of the mitochondrial DNA from beef liver gives the possibility of detecting if the product has been irradiated or not. An identification method could finally be developed. The appraisal of the mitochondrial supercoiled DNA fraction constitutes an unambiguous detection test for beef liver irradiation. A total of 120 different samples were irradiated at 5 different doses. The dose limit of detection is lower than 2 kGy. No effects due to storage conditions were observed.

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