{"title":"在接枝聚乙烯上固定化微粒体和酶","authors":"T.I. Davidenko, S.A. Andronati","doi":"10.1016/0167-6989(88)90048-7","DOIUrl":null,"url":null,"abstract":"<div><p>Immobilization of microsomal fractions, proteolytic enzymes and urease on polyethylene-grafted poly(acrylic acid), poly(allyl alcohol) and poly(allylamine) was investigated. Immobilization of microsomal fractions on polyethylene-grafted poly(allyl alcohol) and poly(acrylic acid) preserved 108 and 31.5% of aniline hydroxylase activity and 125 and 109% of <em>N,N</em>-dimethylaniline demethylase activity, respectively. An increased pH, temperature, and storage stability of the immobilized microsomes was observed. Column operation was performed as well. Positive results were also obtained in the immobilization of urease and <em>Bacillus mesentericus</em> protease.</p></div>","PeriodicalId":101060,"journal":{"name":"Reactive Polymers, Ion Exchangers, Sorbents","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-6989(88)90048-7","citationCount":"0","resultStr":"{\"title\":\"Microsomes and enzymes immobilized on grafted polyethylenes\",\"authors\":\"T.I. Davidenko, S.A. Andronati\",\"doi\":\"10.1016/0167-6989(88)90048-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Immobilization of microsomal fractions, proteolytic enzymes and urease on polyethylene-grafted poly(acrylic acid), poly(allyl alcohol) and poly(allylamine) was investigated. Immobilization of microsomal fractions on polyethylene-grafted poly(allyl alcohol) and poly(acrylic acid) preserved 108 and 31.5% of aniline hydroxylase activity and 125 and 109% of <em>N,N</em>-dimethylaniline demethylase activity, respectively. An increased pH, temperature, and storage stability of the immobilized microsomes was observed. Column operation was performed as well. Positive results were also obtained in the immobilization of urease and <em>Bacillus mesentericus</em> protease.</p></div>\",\"PeriodicalId\":101060,\"journal\":{\"name\":\"Reactive Polymers, Ion Exchangers, Sorbents\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0167-6989(88)90048-7\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reactive Polymers, Ion Exchangers, Sorbents\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0167698988900487\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reactive Polymers, Ion Exchangers, Sorbents","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0167698988900487","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Microsomes and enzymes immobilized on grafted polyethylenes
Immobilization of microsomal fractions, proteolytic enzymes and urease on polyethylene-grafted poly(acrylic acid), poly(allyl alcohol) and poly(allylamine) was investigated. Immobilization of microsomal fractions on polyethylene-grafted poly(allyl alcohol) and poly(acrylic acid) preserved 108 and 31.5% of aniline hydroxylase activity and 125 and 109% of N,N-dimethylaniline demethylase activity, respectively. An increased pH, temperature, and storage stability of the immobilized microsomes was observed. Column operation was performed as well. Positive results were also obtained in the immobilization of urease and Bacillus mesentericus protease.
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