柽柳叶甲醇提取物的抗氧化和抑菌活性

M. C. Kumar, S. A. Kader, C. Sivaraj, N. Raaman
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摘要

摘要本研究旨在评价青叶甲醇提取物的植物化学成分、自由基清除活性、抗氧化性能和抗菌活性。采用标准方法,对柽柳甲醇叶提取物中的植物化学物质进行了定性和定量测定。通过DPPH自由基清除率、OH•自由基清除率、NO•自由基清除率、fe3 +还原能力和磷钼还原试验等方法测定其抗氧化活性。采用孔扩散法测定其抑菌活性。柽柳叶甲醇提取物具有良好的自由基清除能力和还原活性,且还原活性随提取物浓度的增加而增强。研究发现,在100µg和100µg/mL浓度分别为211.0 mg/g和52.91 mg/g的主要植物化学物质,如酚类和类黄酮。本研究表明,柽柳叶甲醇提取物具有显著的抗氧化和抑菌活性。
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Antioxidant and antimicrobial activities of methanol leaf extract of Aegle Tamilnadensis Abdul Kader
The present investigation was aimed to evaluate the phytochemical constituents, free radical scavenging activities, antioxidant properties and antimicrobial activities of the methanol leaf extract of Aegle tamilnadensis Abdul Kader. The phytochemicals present in the methanol leaf extract of A. tamilnadensis Abdul Kader were determined qualitatively and quantitatively using standard procedures. The antioxidant activities were carried out by DPPH free radical scavenging assay, OH • radical scavenging assay, NO • radical scavenging assay, Fe 3+ reducing power assay, and phosphomolybdenum reduction assay methods. The antimicrobial activity was carried out by well diffusion method. The methanol leaf extract of A. tamilnadensis Abdul Kader showed good free radical scavenging as well as reducing power activities which were found to increase with the increasing concentration of the extract. The study revealed the presence of major phytochemicals such as phenols and flavonoids at the concentration of 211.0 mg/g at 100 µg and 52.91 mg/g at 100µg/mL respectively. The present study revealed that the methanol leaf extract of A. tamilnadensis Abdul Kader possess significant antioxidant and antimicrobial activities.
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