Forensic determination of the time of formation of damage to internal human organs using the method of reconstructing the optical activity of histological sections

Purpose of work. Development of a technique for determining the time of formation of damage to human internal organs by digital histological methods of polarization reconstruction of circular birefringence of molecular complexes.Materials and methods. The object of the study was the histology of samples of human internal organs (brain, kidney and liver) with different duration of damage from 1 to 120 hours. For control, we used biological tissue samples of those who died from coronary heart disease with different duration of damage from 1 to 120 hours. The studies were carried out using the technique of polarization reconstruction of circular birefringence of molecular complexes.Results. A set of diagnostically relevant relationships between temporal changes in the statistical structure of topographic maps of circular birefringence of optically active molecular complexes of histological sections of human internal organs with different duration of damage and variations in the mean value, dispersion, asymmetry and excess, characterizing the distribution of the value of this parameter of anisotropy, has been determined.Conclusions. A new original method has been developed for tomography of the optical activity of molecular complexes of tissues of human internal organs in a digital histological study of the age of damage to the tissues of the brain, liver and kidney, as well as the myocardium and lung tissue at a time interval of 1 up to 120 hours.