研究体外受精实验室性能指标维也纳共识2017根据诊断和辅助生殖不育技术高级研究所,Al-Nahrain大学,伊拉克

Zahra A. Hussein, Ali Rahim, Wasan Adnan Abdul-Hameed
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引用次数: 0

摘要

背景:绩效指标用于评估患者的安全性、有效性、公平性、以患者为中心、准时性和效率。每个关键性能指标的基准值为抽吸值,最低性能水平值分别为第1天的受精卵母细胞数量和正常受精率(注射后17小时测量的2Pro核和2极体的存在)。受精率失败计算为第1天(授精后17小时)无妊娠迹象的IVF周期(不包括胞浆内注射周期)的比例。在受精过程中(即0个卵母细胞带有2Pro核)。第2天(受精后44小时)的受精卵百分比被称为卵裂率,它可以表明精子质量(精子功能、卵母细胞激活和配子受体)、精子加工或用于授精的精子数量的问题。分裂产生胚胎。每个成功受精卵在第2天(授精后44小时)处于4细胞阶段或在第3天(授精后68小时)处于8细胞阶段的卵裂胚胎的百分比称为胚胎发育率。这是为了评估胚胎的活力和质量,以及培养系统在必要阶段促进卵裂的能力。关键因素是在授精116小时后观察到的囊胚比例与正确受精的卵母细胞数量的关系。性能指标囊胚发育速率。胚胎的活力以及培养系统支持受精卵母细胞形成囊胚的能力(即细胞内团块和囊胚腔的形成)都是由这个因素决定的。需要注意的是,这个短语只考虑囊胚的形成,而不考虑囊胚的阶段或质量。损伤率是指在第1天受精评估时由于胞浆内注射而损伤或恶化的卵母细胞的比例。活检和管状/固定样本中发现DNA的百分比代表活检的成功率。它可以作为胚胎学家将活检样本转移到试管中的能力的衡量标准,如成功的DNA扩增所示。用胚胎囊数除以移植胚胎总数就可以计算着床率,而着床率取决于卵裂阶段。用妊娠囊数除以移植囊胚总数,计算着床KPI(囊胚期)。
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Studying the IVF Laboratory Performance Indicators the Vienna Consensus 2017 for the High Institute According to Diagnosis and Assisted Reproductive for Infertility Technologies, Al-Nahrain University, Iraq
Background: Performance indicators are used to assess patient safety, efficacy, equity, patient-centeredness, punctuality, and efficiency. The benchmark values for each Key Performance Indicator are aspirational values, and the minimum performance level values are the number of fertilized oocytes on Day 1 and the Normal Fertilization Rate, respectively (presence of 2Pro Nucleus and 2Polar Body measured at 17 h post-injection) as a Failed fertilization rate is calculated as the proportion of IVF cycles (excluding cycles with intracytoplasmic injection) on Day 1 (17 hours after insemination) with no signs of pregnancy. During fertilization (i.e., 0 oocytes with 2Pro Nucleus). The percentage of zygotes on Day 2 (44 hours after insemination) is known as the cleavage rate, and it can suggest an issue with sperm quality (sperm function, oocyte activation, and gamete receptors), sperm processing, or the quantity of spermatozoa used for insemination. which cleaves to create embryos. The percentage of cleaved embryos per successfully fertilized egg that are at the 4-cell stage on Day 2 (44 hours post-insemination) or at the 8-cell stage on Day 3 (68 hours post-insemination) is known as the embryo development rate. This evaluates the viability and quality of the embryos as well as the capacity of the culture system to promote cleavage at the necessary stages. The critical factor is the proportion of blastocysts observed at 116 hours after insemination as a function of the number of correctly fertilized oocytes. Measures of performance blastocyst development rate. The viability of the embryo as well as the culture system's capacity to support blastocyst formation from fertilized oocytes (i.e., the formation of an intracellular mass and a blastocoele cavity) are both determined by this factor. It should be noted that this phrase only considers blastocyst formation and not blastocyst stage or quality. The damage rate is the proportion of oocytes that are injured or have deteriorated by the time of fertilization assessment on Day 1 as a result of the intracytoplasmic injection. The percentage of biopsied and tubed/fixed samples where DNA is found represents the success rate of the biopsy. It serves as a gauge of the embryologists' ability to transfer biopsied samples to test tubes, as shown by successful DNA amplification. The number of gestational sacs divided by the total number of transplanted embryos is how one calculates the implantation rate, which is dependent on the cleavage stage. By dividing the number of gestational sacs by the total number of transplanted blastocysts, the implantation KPI (blastocyst stage) is calculated.
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