木霉对肯尼亚香蕉尖孢镰刀菌有潜在的抑制作用

Samuel Musime Malaka, D. M. Thuranira, M. Mwangi, S. Nchore, Hudson Lubabali, Sylvia W Kuria, Daniel Omingo Omari, C. Gathambiri
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摘要

目的:本研究考察了多菌灵、哈茨木霉、曲霉、绿霉以及多菌灵与生物杀菌剂联合使用在体外和温室条件下对Foc的治理效果。方法与结果:采用中毒食品法进行体外试验。连续7天每天记录真菌径向生长情况。在温室内,试验产品和Foc直接施用于土壤中,每隔7天记录病害严重程度数据,连续98 d,按1-5分进行。试验结束时(98 d)记录植物生物量数据。采用方差分析分析真菌径向生长、疾病严重程度和植物生物量的数据。采用Student-Newman-Keuls (SNK)检验比较,P≤0.05。在体外试验中,与未处理对照组相比,各处理均显著(P<0.05)抑制了Foc的生长。在温室试验中,外黄化症状有显著性差异(P<0.05),但血管变色无显著性差异。结论和研究结果的应用:本研究为生物杀菌剂和多菌灵在管理巴拿马病中的性能提供了见解。关键词:香蕉;尖孢镰刀菌;巴拿马病,木霉属。
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Trichoderma species have potential in suppressing Fusarium oxysporum f.sp cubense infecting banana in Kenya
Objectives: This study examined the effectiveness of carbendazim, Trichoderma harzianum, T. asperellum, T. viride, and a combination of the carbendazim and the bio-fungicides for the management of Foc both under in vitro and greenhouse conditions. Methodology and Results: The poisoned food technique was used in the in vitro tests. Fungal radial growth was recorded daily for seven days. In the greenhouse, the test products and Foc were applied directly into the soil, disease severity data was recorded at seven day intervals for 98 days using a scale of 1-5. Data on plant biomass was recorded at the end of the experiment (98 days). ANOVA was used to analyze data on fungal radial growth, disease severity, and plant biomass. The Student-Newman-Keuls (SNK) test was used to compare the results at P≤0.05. For the in vitro test, all treatments significantly (P<0.05) suppressed the growth of Foc compared to the untreated control. In the greenhouse trials a significant difference (P<0.05) in external/yellowing symptoms was observed but vascular discoloration was not different. Conclusions and application of findings: This study provides insights into the performance of bio fungicides and carbendazim in managing Panama disease. Keywords: Banana, Fusarium oxysporum f.sp. cubense, , Panama disease, Trichoderma sp.
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