琼脂糖凝胶电泳分离的主要血清脂蛋白中胆固醇水平测定公式的建立

S. Vanavanan, Sirirat Chaloeysup, K. Kotani, Pornpen Srisawasdi
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引用次数: 1

摘要

背景/目的:电泳用于检测脂蛋白组分模式。在脂蛋白模式中同时添加每个脂蛋白部分的胆固醇水平可以更有效地帮助临床决策。本研究的目的是在最近的系统中开发估算分级脂蛋白胆固醇水平的公式,琼脂糖凝胶Sebia HYDRAGEL LIPO+Lp(a)电泳。方法:采用两种Sebia电泳法,HYDRAGEL LIPO+Lp(a)法和HYDRAGEL LDL/HDL-CHOL Direct法对血清样品进行分析。利用线性回归模型建立了单个脂蛋白组分相对胆固醇(%)的估算公式。然后,通过将相对胆固醇与总胆固醇浓度相乘计算出的脂蛋白胆固醇值与标准化酶测定值进行比较。结果:高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和极低密度脂蛋白(VLDL)分数的相对胆固醇(y)计算公式分别为y=x-8、x+21和0.75x-6.5。在加Lp(a)和不加Lp(a)的样品中,计算出的测定值(y)与标准化酶法测定值(x)的回归统计量分别为:hdl -胆固醇y=1.07x′- 0.18和1.06x′-0.06,ldl -胆固醇y=0.90x′+0.32和0.92x′+0.29,vldl -胆固醇y=0.85x′-0.03和0.95x′+0.02。结论:所提出的公式可以通过HYDRAGEL LIPO+Lp(a)电泳可靠地估计各主要脂蛋白部分的胆固醇水平。其应用有待进一步的研究。
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Development of Formulas for Estimation of Cholesterol Levels in Major Serum Lipoproteins Separated by Agarose Gel Electrophoresis
Background/aim: Electrophoresis is useful for examining the lipoprotein fraction patterns. A simultaneous and cost-effective addition of cholesterol levels in each lipoprotein fraction to the lipoprotein patterns can more assist clinical decision-making. This study' aim was to develop the formulas for estimating the fractionated lipoproteins cholesterol levels in a recent system, the agarose gel Sebia HYDRAGEL LIPO+Lp(a) electrophoresis. Methods: Serum samples were analyzed by two Sebia electrophoresis, HYDRAGEL LIPO+Lp(a) and the quantitative HYDRAGEL LDL/HDL-CHOL Direct methods. The formulas for estimation of relative cholesterol (%) of individual lipoprotein fractions were developed using linear regression models. Thereafter, the calculated lipoproteins cholesterol values by multiplying the relative cholesterol with total cholesterol concentrations were compared with the standardized enzymatic assayed values. Results: The equations for calculating % relative cholesterol (y) from % relative lipoprotein (x) were y=x-8, x+21 and 0.75x-6.5 for high-density lipoprotein (HDL), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) fractions, respectively. Regression statistics obtained between the calculated assays (y) and the standardized enzymatic assays (x') in samples with and without Lp(a) were y=1.07x'- 0.18 and 1.06x'-0.06, respectively for HDL-cholesterol, y=0.90x'+0.32 and 0.92x'+0.29 for LDL-cholesterol, and y=0.85x'-0.03 and 0.95x'+0.02 for VLDL-cholesterol. Conclusions: The proposed formulas can provide a reliable estimation of cholesterol levels in each major lipoprotein fraction by the HYDRAGEL LIPO+Lp(a) electrophoresis. Further studies with its application are needed.
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