Antibacterial Effect of Platelet Concentrate and Amniotic Membrane as two Human-derived Biological Products

Background and Objectives: Regarding the increasing spread of bacterial resistance, researchers are always interested in finding effective antibiotics of natural origin. The amniotic membrane and blood platelet concentrate are two biological products with an antibacterial effect. The present study aimed to investigate the antibacterial effect of the biological products on broad-spectrum MBL-producing Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus. Subjects and Methods The amniotic membrane, blood platelet concentrate, S. aureus, and P. aeruginosa isolates were collected from hospitals in Gorgan City, Iran. The isolates were identified using the biochemical tests. The methicillin-resistant S. aureus and MBL-producing P. aeruginosa strains were collected by the combined disk and iodometric methods. The antibacterial effects of platelet serial dilutions of bacteria were prepared using 0.5 McFarland turbidity standard suspension in tubes. Then, different concentrations of bacteria were mixed with platelet. After four different encounter durations, a sample was obtained and cultured on medium and bacterial growth was examined. The amniotic membrane was assessed by disk diffusion methods. Results The results showed that all isolates of P. aeruginosa and S. aureus were MBL producers. The platelet concentrate showed the antibacterial effect on all S. aureus isolates, whereas it lacked such an effect on P. aeruginosa isolates. It indicates that the amniotic membrane has an antibacterial effect on all S. aureus and P. aeruginosa isolates. Conclusion The amniotic membrane and platelet concentrate showed high antimicrobial potential against multidrug-resistant S. aureus and P. aeruginosa pathogens. Therefore, human-derived natural products can be used as a source for efficient antibiotics.