三卵黄-幼椰子水稀释杜洛克猪雄精液的超长保质期对其活力、异常值和pH值的影响

Fransiska Luruk Berek, A. A. Dethan, Paulus Klau Tahuk
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摘要

本研究旨在研究用三蛋黄-椰子水稀释剂稀释的杜洛克猪精液的保质期对其活力值、异常值和酸度(pH)的影响。使用的精液是2岁杜洛克猪的新鲜精液。精液采用人工方法采集。本研究于2020年11月采用完全随机设计(CRD)程序的实验方法进行,共4个处理,4个重复,共16个实验单元。各处理分别为:P0:保存0小时,P1:保存24小时,P2:保存36小时,P3:保存48小时。结果表明:三蛋黄-椰子水稀释液的使用对鱼的活力、异常度和酸度(pH)有显著影响(P <0.05)。精子存活率均值P0: 96%, P1: 93.75%, P2: 84.5%, P3: 79.5%。P0组精子异常平均值为5.25%,P1组为6.5%,P2组为11.25%,P3组为14.75%。精液酸度(pH)平均值P0: 8.3 P1: 7.2;P2 7、3和P3处理:7、25。综上所述,用三卵黄和椰子水稀释精液可以维持精子活力值、精子异常值和酸度(pH)。
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The Effect of Long Shelf Life of Duroc Pig Male Semen Diluted Using Tris-Egg Yolk-Young Coconut Water on The Value of Viability, Abnormality and pH
This study aims to determine the effect of shelf life of duroc boar semen which was diluted using tris-egg yolk-coconut water thinner on the value of viability, abnormality, and acidity (pH). The semen used is in the form of fresh semen from 2-year-old duroc pigs. Semen was collected by a manual method using artificial broodstock. This research was conducted in November 2020 using an experimental method according to the Completely Randomized Design (CRD) procedure with 4 treatments and 4 replications so that there were 16 experimental units. Each treatment is P0: storage of semen for 0 hours, P1: storage of semen for 24 hours, P2: storage of semen for 36 hours, P3: storage of semen for 48 hours. The results showed that the use of tris-egg yolk-coconut water diluent had a significant effect (P <0.05) on viability, abnormality, and degree of acidity (pH). The mean value of spermatozoa viability was P0: 96%, P1: 93.75%, P2: 84.5%, and treatment P3: 79.5%. The mean value of spermatozoa abnormality in treatment P0: 5.25%, P1: 6.5%, P2: 11.25%, and treatment P3: 14.75%. The average value of the degree of acidity (pH) of semen P0: 8.3 P1: 7.2; P2 7,3 and P3 treatment: 7,25. It can be concluded that the dilution of semen using tris-egg yolk and coconut water can maintain the value of viability, abnormalities of spermatozoa, and degree of acidity (pH).  
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