{"title":"不同栽培技术对冬豌豆托叶光合色素含量及比值的影响","authors":"I. Didur, V. Shevchuk","doi":"10.37128/2707-5826-2022-2-3","DOIUrl":null,"url":null,"abstract":"The article describes the results of research on the content and ratio of photosynthetic pigments in the stipules of winter peas using different cultivation technologies. The objects of the study were winter pea plants (Pisum sativum L.) of variety NS Moroz, plant growth regulator (PGR) Endofit L1 RK, bacterial preparation Bioinoculant BTU-r, microfertilizers «LF-Bobovi» and Biobor 140. The purpose of our research was to study the formation of photosynthetic pigments of winter pea plants under the action of growth regulator Endophyte L1 RK, bacterial preparation Bioinoculant BTU-r, their complex applying and foliar treatment systems with microfertilizers «LF- Bobovi» and Biobor 140. Analysis of chlorophyll and carotenoid content in alcohol extract (96.0%) was performed using a spectrophotometer Ulab − 102UV (China) at different values of wavelength: λ = 441; λ = 649; λ = 665. The volume of the working solution was 25 ml. Calculations of these indexes were performed according to the formulas of H. K. Lichtenthaler. During the study period, the influence of plant growth regulator, bacterial preparation, and foliar fertilization on the content of chlorophyll a and b, their sum, and concentration of carotenoids in the stipules of winter pea plants were evaluated. The research results indicate the dependence of these indicators on the use of the growth regulator and the bacterial drug, as well as on foliar fertilization. It was found that the implementation of foliar treatment in the phase of 3-5 stipules and complex use (in phases 3-5 of stipules and buttons) phase with tank mixtures of micro fertilizers «LF- Bobovi» (1.5 l / ha and 2.5 l / ha) and Biobor 140 (1.0 l / ha) led to an increase of the content of chlorophyll a and b, their total amount and carotenoid content. The highest content of chlorophylls a and b, their sum, and carotenoids in the stipules of winter pea plants were in the variant with the combined use of pre-sowing seed treatment with growth regulator Endophyte L1 RK (0.01 l / t), bacterial preparation Bioinoculant BTU-r (2 l / t) and at foliar treatment with microfertilizers «LF- Bobovi» (1.5 l / ha and 2.5 l / ha) and Biobor 140 (1.0 l / ha).The search for ways and opportunities to increase the grain yield of winter pea varieties Moroz due to the introduction of new technological methods of its cultivation remains relevant. A possible solution to the problem of improving the productivity of winter peas is the use of different mechanisms of action of plant growth regulators, inoculation with symbiotic microorganisms, and foliar feeding with tank mixtures of chelated microfertilizers.","PeriodicalId":7511,"journal":{"name":"Agriculture and Forestry","volume":"76 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CONTENT AND RATIO OF PHOTOSYNTHETIC PIGMENTS IN STIPULES OF WINTER PEA WITH USING DIFFERENT GROWING TECHNOLOGIES\",\"authors\":\"I. Didur, V. Shevchuk\",\"doi\":\"10.37128/2707-5826-2022-2-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The article describes the results of research on the content and ratio of photosynthetic pigments in the stipules of winter peas using different cultivation technologies. The objects of the study were winter pea plants (Pisum sativum L.) of variety NS Moroz, plant growth regulator (PGR) Endofit L1 RK, bacterial preparation Bioinoculant BTU-r, microfertilizers «LF-Bobovi» and Biobor 140. The purpose of our research was to study the formation of photosynthetic pigments of winter pea plants under the action of growth regulator Endophyte L1 RK, bacterial preparation Bioinoculant BTU-r, their complex applying and foliar treatment systems with microfertilizers «LF- Bobovi» and Biobor 140. Analysis of chlorophyll and carotenoid content in alcohol extract (96.0%) was performed using a spectrophotometer Ulab − 102UV (China) at different values of wavelength: λ = 441; λ = 649; λ = 665. The volume of the working solution was 25 ml. Calculations of these indexes were performed according to the formulas of H. K. Lichtenthaler. During the study period, the influence of plant growth regulator, bacterial preparation, and foliar fertilization on the content of chlorophyll a and b, their sum, and concentration of carotenoids in the stipules of winter pea plants were evaluated. The research results indicate the dependence of these indicators on the use of the growth regulator and the bacterial drug, as well as on foliar fertilization. It was found that the implementation of foliar treatment in the phase of 3-5 stipules and complex use (in phases 3-5 of stipules and buttons) phase with tank mixtures of micro fertilizers «LF- Bobovi» (1.5 l / ha and 2.5 l / ha) and Biobor 140 (1.0 l / ha) led to an increase of the content of chlorophyll a and b, their total amount and carotenoid content. The highest content of chlorophylls a and b, their sum, and carotenoids in the stipules of winter pea plants were in the variant with the combined use of pre-sowing seed treatment with growth regulator Endophyte L1 RK (0.01 l / t), bacterial preparation Bioinoculant BTU-r (2 l / t) and at foliar treatment with microfertilizers «LF- Bobovi» (1.5 l / ha and 2.5 l / ha) and Biobor 140 (1.0 l / ha).The search for ways and opportunities to increase the grain yield of winter pea varieties Moroz due to the introduction of new technological methods of its cultivation remains relevant. 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引用次数: 0
摘要
本文介绍了不同栽培技术对冬豌豆托叶光合色素含量和比例的研究结果。以NS Moroz品种冬豌豆(Pisum sativum L.)、植物生长调节剂(PGR) Endofit L1 RK、细菌制剂生物接种剂BTU-r、微量肥料LF-Bobovi和Biobor 140为研究对象。本研究旨在研究生长调节剂内生菌L1 RK、细菌制剂生物接种剂BTU-r及其复合施用和微肥“LF- Bobovi”和Biobor 140叶面处理体系对冬豌豆光合色素形成的影响。用Ulab−102UV分光光度计(中国)在不同波长值下分析醇提物中叶绿素和类胡萝卜素含量(96.0%):λ = 441;λ = 649;λ = 665。工作液的体积为25 ml。这些指标的计算依据H. K. Lichtenthaler公式。在研究期间,研究了植物生长调节剂、细菌制剂和叶面施肥对冬豌豆植株托叶叶绿素a和b含量、叶绿素a和叶绿素b总量以及类胡萝卜素浓度的影响。研究结果表明,这些指标依赖于生长调节剂和细菌药物的使用,以及叶面施肥。结果表明,在托叶3 ~ 5期进行叶面处理,在托叶3 ~ 5期复合施用“LF- Bobovi”(1.5 l / ha和2.5 l / ha)和“Biobor 140”(1.0 l / ha)混合施用,可提高叶绿素a和叶绿素b的含量、总含量和类胡萝卜素含量。套用生长调节剂内生菌L1 RK (0.01 l / t)、细菌制剂生物接种剂BTU-r (2 l / t)和叶面微肥“LF- Bobovi”(1.5 l / ha和2.5 l / ha)和Biobor 140 (1.0 l / ha)处理的冬豌豆植株托叶叶绿素a和叶绿素b含量、总含量和类胡萝卜素含量最高。由于引进了新的栽培技术方法,寻找方法和机会来提高冬豌豆品种莫罗兹的籽粒产量仍然是有意义的。提高冬豌豆产量的一个可能的解决办法是利用植物生长调节剂的不同作用机制,接种共生微生物,以及用螯合微肥料的罐混合物在叶面上饲喂。
CONTENT AND RATIO OF PHOTOSYNTHETIC PIGMENTS IN STIPULES OF WINTER PEA WITH USING DIFFERENT GROWING TECHNOLOGIES
The article describes the results of research on the content and ratio of photosynthetic pigments in the stipules of winter peas using different cultivation technologies. The objects of the study were winter pea plants (Pisum sativum L.) of variety NS Moroz, plant growth regulator (PGR) Endofit L1 RK, bacterial preparation Bioinoculant BTU-r, microfertilizers «LF-Bobovi» and Biobor 140. The purpose of our research was to study the formation of photosynthetic pigments of winter pea plants under the action of growth regulator Endophyte L1 RK, bacterial preparation Bioinoculant BTU-r, their complex applying and foliar treatment systems with microfertilizers «LF- Bobovi» and Biobor 140. Analysis of chlorophyll and carotenoid content in alcohol extract (96.0%) was performed using a spectrophotometer Ulab − 102UV (China) at different values of wavelength: λ = 441; λ = 649; λ = 665. The volume of the working solution was 25 ml. Calculations of these indexes were performed according to the formulas of H. K. Lichtenthaler. During the study period, the influence of plant growth regulator, bacterial preparation, and foliar fertilization on the content of chlorophyll a and b, their sum, and concentration of carotenoids in the stipules of winter pea plants were evaluated. The research results indicate the dependence of these indicators on the use of the growth regulator and the bacterial drug, as well as on foliar fertilization. It was found that the implementation of foliar treatment in the phase of 3-5 stipules and complex use (in phases 3-5 of stipules and buttons) phase with tank mixtures of micro fertilizers «LF- Bobovi» (1.5 l / ha and 2.5 l / ha) and Biobor 140 (1.0 l / ha) led to an increase of the content of chlorophyll a and b, their total amount and carotenoid content. The highest content of chlorophylls a and b, their sum, and carotenoids in the stipules of winter pea plants were in the variant with the combined use of pre-sowing seed treatment with growth regulator Endophyte L1 RK (0.01 l / t), bacterial preparation Bioinoculant BTU-r (2 l / t) and at foliar treatment with microfertilizers «LF- Bobovi» (1.5 l / ha and 2.5 l / ha) and Biobor 140 (1.0 l / ha).The search for ways and opportunities to increase the grain yield of winter pea varieties Moroz due to the introduction of new technological methods of its cultivation remains relevant. A possible solution to the problem of improving the productivity of winter peas is the use of different mechanisms of action of plant growth regulators, inoculation with symbiotic microorganisms, and foliar feeding with tank mixtures of chelated microfertilizers.