斑马草甲醇提取物中酚类生物活性物质的测定及抗氧化和溶血活性的评价

Vagner Cardoso Da Silva, B. E. A. D. MagalhÃes, Tatiana Barbosa dos Santos Magalhães, E. T. Guimarães, Alessandra Aa Silva Guedes, M. D. Mota, W. D. dos Santos, B. A. Cerqueira, A. F. S. Santos Júnior
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摘要

摘要:斑马草(Tradescantia zebrina)是一种主要生长于南美洲的植物,目前关于其化学成分和生物活性的报道较少。目的:首次在文献中测定其酚类生物活性成分,并测定其提取物(干燥提取物和天然提取物)的抗氧化和溶血活性。方法:提取液经甲醇浸出,总酚和总黄酮含量分别以提取物的没食子酸(EAG)/g和槲皮素(EQ) mg当量表示。采用DPPH•和ABTS•+自由基捕获法评价其抗氧化能力。采用高效液相色谱耦合二极管检测器(HPLC-DAD)对酚类生物活性化合物进行鉴定和定量,采用圆盘抗菌素技术在血琼脂中扩散进行溶血活性测定。结果:天然提取物和干燥提取物的总酚和总黄酮含量分别为67.68和233.94 mg EAG/g,浓缩提取物的总酚和总黄酮含量分别为29.70和10.99 mg EQ/g。采用高效液相色谱-双阵列检测器(HPLC-DAD)测定酚酸(咖啡酸、鞣花酸、阿魏酸、ρ-香豆酸和原儿茶酸)、黄酮类(大黄素和芦丁)和酚醛(香兰素)。对DPPH•(44.08%)和ABTS•+(46.99%)自由基的抑制作用证实了其抗氧化活性。结论:经72 h溶血活性测定,莪术醇提物无毒性。因此,斑马草是生物活性物质的潜在来源
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Determination of phenolic bioactive compounds and evaluation of the antioxidant and hemolytic activities in the methanolic extracts of Tradescantia zebrina
Introduction: Tradescantia zebrina is a species used mainly in South America, with few reports about its chemical composition and bioactivity. Aim: To determine phenolic bioactive compounds, for the first time in the literature, and the antioxidant and hemolytic activities of extracts (dried and in natura) of this species. Methods: The extracts were obtained by maceration in methanol and the total phenolic and flavonoid contents were expressed in mg equivalent of gallic acid (EAG)/g and quercetin (EQ) of extract, respectively. The antioxidant potential was evaluated by DPPH• and ABTS•+ radical capture methods. The identification and quantification of phenolic bioactive compounds was performed by high performance liquid chromatography coupled to a diode detector (HPLC-DAD) and, the hemolytic activity assay was performed by diffusion in blood agar, using the disk antiobiogram technique. Results: The total phenolic and flavonoid contents in the extracts (in natura and dried) were 67.68 and 233.94 mg EAG/g and 29.70 and 10.99 mg EQ/g of concentrated extract, respectively. Phenolic acids (caffeic, ellagic, ferulic, ρ-coumaric and protocatechuic), flavonoids (chrysin and rutin) and a phenolic aldehyde (vanillin) were determined by high performance liquid chromatography-diodearray detector (HPLC-DAD). The antioxidant activities were confirmed by inhibition of DPPH• (44.08%) and ABTS•+ (46.99%) radicals for in natura samples, respectively. Conclusion: The tested methanolic extracts of Tradescantia zebrina did not present toxicities when tested for hemolytic activity after 72 hours. Therefore, Tradescantia zebrina is a potential source of bioactive substances    
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