Relationship Between Calving Rate and Concentration of Hormones and Blood Metabolites During Pregnancy in Post-Induction Pasundan Cows GnRH

Twenty Pasundan heifers were used in the study to find out calving rates related to the concentration of hormones and metabolites of the mother's blood during the farm. The experimental mother cow was given a combination of the hormone prostaglandin F2α as much as 5 ml per head and gonadotropin realising hormone (GnRH) of 2.5 ml per head intramuscular to uniformize fertility conditions and improve fertility. The mother cow is immediately carried out artificial insemination 2 times with an interval of 6 hours. All experimental cows were given forage basalt food and adlibitum drinking water. Pregnancy examination is carried out on the 60th and 150th days of post-insemination using the rectal palpation method. The observed variables in the form of calving rate were related to plasma concentrations of the hormones progesterone (P), estrogen (E), and blood metabolites levels β-OH butyric (BHBA), blood urea nitrogen (BUN), and non-esterified fatty acids (NEFA). The results showed that in Garut calving average rate of 90%, the plasma concentration of hormone progesterone 4.73±0.28 ng/ml and estrogen 24.59±1.91 pg/ml higher than Bogor with an average calving rate of 70%, the plasma concentration of progesterone hormone 3.46±0.71ng/ml and estrogen 21.67±1.57 pg/ml. The concentration of BHBA, BUN, and NEFA for Bogor region respectively was 12.33±1.81 mg dl-1; 23.70±2.40 mg dL; 1.85±0.25 mmol L-1) higher than Garut in a row is 10.17±1.25*mg dl-1, 22.70±2.70mg dL; 1.76±0.37mmol L-1). The results of regression analysis showed that the relationship of calving rate (CR) with the concentration of hormones conceptus estrogen (E) and progesterone (P) in garut region has a real form of linear relationship (P<0.05) by following the equation Calving rate=4772 +0.232 Estrogen + 0.643 Progesterone - 0.074 BHBA + 0.335 NEFA - 0.082 BUN with a coefficient of determination of 43.65%, while for Bogor region according to Calving regression rate=5,590+ 0.010 Estrogen +0.638 Progesterone - 0.231 BHBA + 1.11NEFA – 0.105 BUN with a coefficient of determination of 16.34%. It was concluded that calving rate is strongly influenced by the concentration of the hormones progesterone and estrogen, as well as having a close relationship with the condition of the mother blood metabolite during the pregnancy.