非变性聚丙烯酰胺凝胶电泳法评价血脂异常受试者小密度低密度脂蛋白相关参数:参照日本预防动脉粥样硬化性心血管疾病指南

K. Tsuzaki, K. Kotani, Kazunori Yamada, Y. Sano, Yukiyo Matsuoka, Kentaro Okazaki, Toshiyuki Yamada, A. Shimatsu, N. Sakane
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引用次数: 1

摘要

为了预防动脉粥样硬化性疾病的发展,临床参考日本动脉粥样硬化性心血管疾病预防指南(2007年日本动脉粥样硬化学会发布)。此外,人们还提倡不仅要评价低密度脂蛋白(LDL)的水平,而且要评价其质量。小密度LDL (sdLDL)被认为是LDL质量的代表。使用lipprinttm LDL亚组分系统,利用非变性聚丙烯酰胺凝胶电泳方法,可以评估sdLDL相关的几个参数,如sdLDL的面积百分比、平均LDL粒径(mean LDL- ps)、相对漂浮率(Rf)形式的相对迁移率,以及LDL基于粒径的表型模式。我们试图根据sdldl相关参数评估日本预防动脉粥样硬化性心血管疾病指南。我们研究了46名无症状和无心血管疾病的血脂异常受试者(23名男性,年龄30-58岁;23名妇女,31-58岁),处于初级预防水平。从指南中增加的类别数量来看,可以观察到以下趋势:LDL-PS越小(p=0.002), sdLDL面积百分比越大(p=0.042), Rf增加(p=0.068), B型(动脉粥样硬化表型)的存在增加(p=0.031)。这些结果提示,使用lipprinttm系统,日本指南在临床上可以很好地反映sdLDL相关参数,特别是平均LDL-PS、sdLDL面积百分比和B型。
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The parameters related to small dense low-density lipoprotein evaluated by non-denaturing polyacrylamide gel electrophoresis method in dyslipidemic subjects : with reference to the Japanese guideline for prevention of atherosclerotic cardiovascular diseases
To prevent the development of atherosclerotic diseases, the Japanese guideline for prevention of atherosclerotic cardiovascular diseases (released from Japan Atherosclerosis Society in 2007) is clinically referred. In addition, the importance of evaluating not only the level but also the quality of low-density lipoprotein (LDL) has been advocated. Small dense LDL (sdLDL) is regarded as representation of the quality of LDL. With the LipoprintTM LDL subfraction system, utilizing non-denaturing polyacrylamide gel electrophoresis method which can evaluate several sdLDL-related parameters such as the area percentage of sdLDL, mean LDL particle size (mean LDL-PS), relative mobility in the form of the relative flotation rate (Rf), and LDL size-based phenotypic patterns. We attempted to assess the Japanese guideline for prevention of atherosclerotic cardiovascular diseases in terms of sdLDL-related parameters. We studied with 46 asymptomatic and cardiovascular disease-free dyslipidemic subjects (23 men, aged 30-58 years olds; 23 women, 31-58 years olds), who were within the primary prevention level. From the increased number of categories in the guideline, the following trends were observed; the smaller LDL-PS (p=0.002), the larger of area percentage of sdLDL (p=0.042), an increase in Rf (p=0.068), and an increase in the presence of pattern B (atherogenic phenotype) (p=0.031). These results suggested that, with the LipoprintTM system, the usage of Japanese guideline may clinically reflect well the sdLDL-related parameters, in particular the mean LDL-PS, the area percentage of sdLDL and the pattern B.
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