Fe Element Analysis in the Spleen Tissue by Using EDS and ICP-MS after Aminated Silica Shelled Magnetite (Fe₃O₄) Administered to the Tail Vein of Mice

The suppression of cytokine storm in severe coronavirus disease 2019 (COVID-19) patients can be treated with monoclonal antibody therapy against CD3 for T cell receptor inhibition. An optimized liquid phase as a CD3 antibody-magnetic nanoparticle (Ab-MNP) conjugate can inhibit the overactivation of T cells. We aim to ana-lyze the distribution of Fe in the spleen after acute administration of silica-conjugated amine magnetite (Fe3O4) nanoparticles (35 nm) delivered by intravenous injection. The Fe element distribution and concentration levels in spleen tissue were analyzed using energy dispersive spectroscopy (EDS) and inductively coupled plasma-mass spectrometry (ICP-MS). The experimental result is a difference in the concentration of Fe elements, which was 1.89×103 mg/kg in the spleen of a control mouse not administered with MNPs, whereas increases sig-nificantly to 1.93×103 mg/kg in that of a mouse administered with MNPs. Further, time kinetic analysis of bio-chemical and immunological parameters is required to confirm its suitability in bio-administration. © 2022 Journal of Magnetics.