{"title":"脂肪细胞疗法在人声带褶皱瘢痕体外纤维生成模型中的旁分泌效应","authors":"Mélanie Velier , Alexia Mattei , Stéphanie Simoncini , Jérémy Magalon , Laurent Giraudo , Laurent Arnaud , Antoine Giovanni , Francoise Dignat-George , Florence Sabatier , Markus Gugatschka , Tanja Grossmann","doi":"10.1016/j.jvoice.2022.05.012","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives/Hypothesis</h3><div>Vocal folds<span> (VF) scarring leads to severe dysphonia<span> which negatively impacts daily life of patients. Current therapeutic options are limited due in large part to the high complexity of the micro-structure of the VF. Innovative therapies derived from adipose tissue such as stromal vascular fraction (SVF) or adipose derived stromal/ stem cells (ASC) are currently being evaluated in this indication and paracrine anti-fibrotic effects are considered as predominant mechanisms.</span></span></div></div><div><h3>Methods</h3><div><span>The paracrine anti-fibrotic effects of SVF and ASC from healthy donors were tested in an innovative </span><em>in vitro</em><span><span><span> fibrogenesis<span> model employing human VF fiboblasts (hVFF) and the principles of macromolecular crowding (MMC). </span></span>Biosynthesis<span><span> of collogen and alpha-smooth-muscle actin (αSMA) expression in hVFF were quantified after five days of indirect coculture<span> with ASC or SVF using </span></span>silver stain, </span></span>western blot and RT-qPCR analysis.</span></div></div><div><h3>Results</h3><div><span>Fibrogenesis was promoted by addition of transforming growth factor beta 1<span> (TGFβ1) combined with MMC characterized by an enhanced deposition of fibrillar collagens and the acquisition of a </span></span>myofibroblast phenotype (overexpression of αSMA). Adipose-derived therapies led to a reduction in the αSMA expression and the collagen content was lower in hVFF co-cultivated with SVF.</div></div><div><h3>Conclusions</h3><div><span>ASC and SVF promoted significant prevention of fibrosis in an </span><em>in vitro</em><span> fibrogenesis model through paracrine mechanisms, supporting further development of adipose-derived cellular therapies in VF scarring.</span></div></div>","PeriodicalId":49954,"journal":{"name":"Journal of Voice","volume":"38 6","pages":"Pages 1259-1263"},"PeriodicalIF":2.5000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Paracrine Effects of Adipose-Derived Cellular Therapies in an in Vitro Fibrogenesis Model of Human Vocal Fold Scarring\",\"authors\":\"Mélanie Velier , Alexia Mattei , Stéphanie Simoncini , Jérémy Magalon , Laurent Giraudo , Laurent Arnaud , Antoine Giovanni , Francoise Dignat-George , Florence Sabatier , Markus Gugatschka , Tanja Grossmann\",\"doi\":\"10.1016/j.jvoice.2022.05.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives/Hypothesis</h3><div>Vocal folds<span> (VF) scarring leads to severe dysphonia<span> which negatively impacts daily life of patients. Current therapeutic options are limited due in large part to the high complexity of the micro-structure of the VF. Innovative therapies derived from adipose tissue such as stromal vascular fraction (SVF) or adipose derived stromal/ stem cells (ASC) are currently being evaluated in this indication and paracrine anti-fibrotic effects are considered as predominant mechanisms.</span></span></div></div><div><h3>Methods</h3><div><span>The paracrine anti-fibrotic effects of SVF and ASC from healthy donors were tested in an innovative </span><em>in vitro</em><span><span><span> fibrogenesis<span> model employing human VF fiboblasts (hVFF) and the principles of macromolecular crowding (MMC). </span></span>Biosynthesis<span><span> of collogen and alpha-smooth-muscle actin (αSMA) expression in hVFF were quantified after five days of indirect coculture<span> with ASC or SVF using </span></span>silver stain, </span></span>western blot and RT-qPCR analysis.</span></div></div><div><h3>Results</h3><div><span>Fibrogenesis was promoted by addition of transforming growth factor beta 1<span> (TGFβ1) combined with MMC characterized by an enhanced deposition of fibrillar collagens and the acquisition of a </span></span>myofibroblast phenotype (overexpression of αSMA). Adipose-derived therapies led to a reduction in the αSMA expression and the collagen content was lower in hVFF co-cultivated with SVF.</div></div><div><h3>Conclusions</h3><div><span>ASC and SVF promoted significant prevention of fibrosis in an </span><em>in vitro</em><span> fibrogenesis model through paracrine mechanisms, supporting further development of adipose-derived cellular therapies in VF scarring.</span></div></div>\",\"PeriodicalId\":49954,\"journal\":{\"name\":\"Journal of Voice\",\"volume\":\"38 6\",\"pages\":\"Pages 1259-1263\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Voice\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S089219972200145X\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AUDIOLOGY & SPEECH-LANGUAGE PATHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Voice","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S089219972200145X","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AUDIOLOGY & SPEECH-LANGUAGE PATHOLOGY","Score":null,"Total":0}
Paracrine Effects of Adipose-Derived Cellular Therapies in an in Vitro Fibrogenesis Model of Human Vocal Fold Scarring
Objectives/Hypothesis
Vocal folds (VF) scarring leads to severe dysphonia which negatively impacts daily life of patients. Current therapeutic options are limited due in large part to the high complexity of the micro-structure of the VF. Innovative therapies derived from adipose tissue such as stromal vascular fraction (SVF) or adipose derived stromal/ stem cells (ASC) are currently being evaluated in this indication and paracrine anti-fibrotic effects are considered as predominant mechanisms.
Methods
The paracrine anti-fibrotic effects of SVF and ASC from healthy donors were tested in an innovative in vitro fibrogenesis model employing human VF fiboblasts (hVFF) and the principles of macromolecular crowding (MMC). Biosynthesis of collogen and alpha-smooth-muscle actin (αSMA) expression in hVFF were quantified after five days of indirect coculture with ASC or SVF using silver stain, western blot and RT-qPCR analysis.
Results
Fibrogenesis was promoted by addition of transforming growth factor beta 1 (TGFβ1) combined with MMC characterized by an enhanced deposition of fibrillar collagens and the acquisition of a myofibroblast phenotype (overexpression of αSMA). Adipose-derived therapies led to a reduction in the αSMA expression and the collagen content was lower in hVFF co-cultivated with SVF.
Conclusions
ASC and SVF promoted significant prevention of fibrosis in an in vitro fibrogenesis model through paracrine mechanisms, supporting further development of adipose-derived cellular therapies in VF scarring.
期刊介绍：
The Journal of Voice is widely regarded as the world''s premiere journal for voice medicine and research. This peer-reviewed publication is listed in Index Medicus and is indexed by the Institute for Scientific Information. The journal contains articles written by experts throughout the world on all topics in voice sciences, voice medicine and surgery, and speech-language pathologists'' management of voice-related problems. The journal includes clinical articles, clinical research, and laboratory research. Members of the Foundation receive the journal as a benefit of membership.
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