Y. Kawakami, H. Iwano, Y. Hatakeyama, Tadashi Inoue, E. Canning, R. Ishihara
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引用次数: 8
摘要
利用KA101和KAI02对引物进行PCR特异性扩增(KAWAKAMI et al., 1995),对其区分微孢子虫属(vairrimorpha属、Pleistophora属和Nosema属)的能力进行了重新研究。目前所检测的小孢子虫,三种小孢子虫和一种小孢子虫的DNA样本,均未能用这些引物产生PCR产物。朱砂蛾的微孢子虫、apis微孢子虫、furnaca/is微孢子虫、mylittensis微孢子虫和tyriae微孢子虫也未产生任何产物。从印度北部(由A. K. Bansal博士提供)、印度南部(由KIYOsHI KAWAKAMI博士提供)和中国(由X. CHEN博士提供)感染微孢子虫的家蚕中分离出的微孢子虫寄生虫通过KA101和KA102引物获得PCR产物。本研究结果表明,利用KA101和KA102引物进行PCR检测,在微晶检测中可用于区分高毒力家蚕菌株。
Use of PCR with the specific primers for discrimination of Nosema bombycis
The specific amplification by PCR using a pair of primers, KA101 and KAI02 (KAWAKAMI et al., 1995) was reinvestigated as to its ability to distinguish microsporidia belonging to genus Vairimorpha, Pleistophora and Nosema. DNA samples from microsporidia of genus Vairimorpha or Pleistophora so far tested, three species of Vairimorpha and one Pleistophora, failed to produce PCR products by those primers. Of Nosema spp., N. apis, N. furnaca/is, N. mylittensis and Nosema tyriae from cinnabar moth, Tyria jacobeae did not also produce any product. Microsporidian parasites isolated from silkworms infected with pebrine in the northern part of India (courtesy of Dr. A. K. Bansal) and in southern India (courtesy of Dr. KIYOsHI KAWAKAMI) and in China (courtesy of Dr. X. CHEN) gave PCR products by KA101 and KA102 primers. The results from our study suggest that PCR tests with the use of KA101 and KA102 primers are useful to distinguish highly virulent strains of N. bombycis in pebrine inspection.