酶修饰丝网印刷电极测定发酵培养基中的葡萄糖,乙醇,乳酸和淀粉

S. Kamanin, V. A. Arlyapov, V. A. Alferov, A. Reshetilov
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引用次数: 4

摘要

将葡萄糖氧化酶、乙醇氧化酶、乳酸氧化酶和γ-淀粉酶固定于交联牛血清白蛋白凝胶中,制备了用于发酵过程监测的丝网印刷电极。确定了基于所研制电极的生物传感器的分析和计量特性。对发酵产物和酒精制品进行了分析。统计分析结果显示,采用多通道系统和参考法测定的葡萄糖、乙醇、乳酸和淀粉含量差异不显著。
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Enzyme-Modified Screen-Printed Electrodes for Assaying Glucose, Ethanol, Lactate and Starch in Fermentation Media
Screen-printed electrodes modified by glucose oxidase, alcohol oxidase, lactate oxidase and γ-amylase immobilized into cross linked bovine serum albumin gel were produced for application in monitoring fermentation processes. The analytical and metrological characteristics of biosensors based on the developed electrodes were determined. Samples of fermentation mass and alcohol products were assayed. Statistical analysis of the results showed the values of glucose, ethanol, lactate and starch contents, determined using the developed multi-channel system and by reference methods, to differ insignificantly.
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