基于核壳的LC定量分离巴基斯坦水飞蓟(Silybum Marianum L.)生态型水飞蓟素变异及其抗氧化活性的单实验室验证

Samantha Drouet, B. Abbasi, Annie Falguiéres, W. Ahmad, Sumaira, C. Ferroud, J. Doussot, Jean-Raymond Vanier, É. Lainé, Christophe Hano
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引用次数: 37

摘要

水飞蓟(L.)果实植物是杉木素类黄酮木脂素的主要来源。这些分子一起构成了一种叫做水飞蓟素的混合物,在化妆品和制药工业中有许多有用的应用。本文建立了一种有效的水飞蓟素成分分离方法,以确保其定量的精密度和准确性。每个化合物的分离具有高重现性。根据AOAC的建议,验证了定量方法的精密度和重复性。并应用该方法研究了野参的自然变异。对这12份来自巴基斯坦的材料果实组成的变异分析证明了巨大的自然多样性。相关分析表明,铜离子还原抗氧化能力(CUPRAC)和铁离子还原抗氧化能力(FRAP)测定表明,不同黄酮木脂素具有协同作用,达到最大的抗氧化活性。主成分分析(PCA)将12份材料分为3个不同的水飞蓟素含量组,而层次聚类分析(HCA)则证实了水飞蓟素成分的强烈变化,从而鉴定出新的富含水飞蓟素的化学型。结果表明,本方法可以有效地分离和定量具有较强抗氧化活性的主要黄酮木脂素。
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Single Laboratory Validation of a Quantitative Core Shell-Based LC Separation for the Evaluation of Silymarin Variability and Associated Antioxidant Activity of Pakistani Ecotypes of Milk Thistle (Silybum Marianum L.)
Fruits of Silybum marianum (L.) Gaernt are the main source of taxifolin derived flavonolignans. Together, these molecules constitute a mixture called silymarin with many useful applications for cosmetic and pharmaceutic industries. Here, a validated method for the separation of the silymarin constituents has been developed to ensure precision and accuracy in their quantification. Each compound was separated with a high reproducibility. Precision and repeatability of the quantification method were validated according to the AOAC recommendations. The method was then applied to study the natural variability of wild accessions of S. marianum. Analysis of the variation in the fruits composition of these 12 accessions from Pakistan evidenced a huge natural diversity. Correlation analysis suggested a synergistic action of the different flavonolignans to reach the maximal antioxidant activity, as determined by cupric ion reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP) assays. Principal component analysis (PCA) separated the 12 accessions into three distinct groups that were differing from their silymarin contents, whereas hierarchical clustering analysis (HCA) evidenced strong variations in their silymarin composition, leading to the identification of new silybin-rich chemotypes. These results proved that the present method allows for an efficient separation and quantification of the main flavonolignans with potent antioxidant activities.
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