{"title":"从腹部浅层和深层皮下脂肪中获取的脂肪干细胞的生成和生长:一个案例","authors":"Daegu Son","doi":"10.4172/2157-7552.1000185","DOIUrl":null,"url":null,"abstract":"A 60 year old woman was enrolled in our study. She was 151 cm tall, weighed 73 kg and had high body mass index (33.19 kg/m2). Adipose tissue specimens were taken from her abdomen by surgical excision. ASCs were isolated in primary culture and cell counts were performed by using an automated cell counter (LUNA™). And 5 × 103 ASCs were seeded into DMEM tissue culture flasks and were cultured for 16 days (n=3). Lastly, self-renewal capacity was demonstrated by colony-forming unit fibroblast (CFUF) assays (n=3).","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"96 1","pages":"1-2"},"PeriodicalIF":0.0000,"publicationDate":"2016-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Yielding and Growing of Adipose Stem Cell Harvested from the Superficial and Deep Abdominal Subcutaneous Fat: A Case\",\"authors\":\"Daegu Son\",\"doi\":\"10.4172/2157-7552.1000185\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A 60 year old woman was enrolled in our study. She was 151 cm tall, weighed 73 kg and had high body mass index (33.19 kg/m2). Adipose tissue specimens were taken from her abdomen by surgical excision. ASCs were isolated in primary culture and cell counts were performed by using an automated cell counter (LUNA™). And 5 × 103 ASCs were seeded into DMEM tissue culture flasks and were cultured for 16 days (n=3). Lastly, self-renewal capacity was demonstrated by colony-forming unit fibroblast (CFUF) assays (n=3).\",\"PeriodicalId\":17539,\"journal\":{\"name\":\"Journal of Tissue Science and Engineering\",\"volume\":\"96 1\",\"pages\":\"1-2\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-11-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Tissue Science and Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/2157-7552.1000185\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Tissue Science and Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2157-7552.1000185","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
一位60岁的妇女参加了我们的研究。她身高151厘米，体重73公斤，身体质量指数高(33.19公斤/平方米)。手术切除腹部脂肪组织标本。在原代培养中分离ASCs，使用自动细胞计数器(LUNA™)进行细胞计数。将5 × 103株ASCs接种于DMEM组织培养瓶中，培养16 d (n=3)。最后，通过集落形成单位成纤维细胞(CFUF)试验证明了自我更新能力(n=3)。
Yielding and Growing of Adipose Stem Cell Harvested from the Superficial and Deep Abdominal Subcutaneous Fat: A Case
A 60 year old woman was enrolled in our study. She was 151 cm tall, weighed 73 kg and had high body mass index (33.19 kg/m2). Adipose tissue specimens were taken from her abdomen by surgical excision. ASCs were isolated in primary culture and cell counts were performed by using an automated cell counter (LUNA™). And 5 × 103 ASCs were seeded into DMEM tissue culture flasks and were cultured for 16 days (n=3). Lastly, self-renewal capacity was demonstrated by colony-forming unit fibroblast (CFUF) assays (n=3).
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