Akp3基因敲除小鼠肝脏肠型碱性磷酸酶mRNA的表达

M. Goseki‐Sone, N. Sogabe, Takanari Nakano, R. Tanabe, M. Haraikawa, D. Alpers, T. Komoda
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引用次数: 2

摘要

在此之前,我们发现高脂喂养大鼠肝脏中肠型碱性磷酸酶(ALP)表达增强。在这里,我们验证了假说,即肠型ALP (Akp3或Akp6)也可能在高脂肪喂养的Akp3-/-小鼠的肝脏中受到调节。以C57Bl/6J雄性小鼠Akp3-/-、Akp3+/-和野生型小鼠分别饲喂正常对照和高脂饲料,观察其对肝脏肠型ALP mRNA的影响。我们发现Akp3 mRNA只在肠道中表达,在肝脏中不表达,而Akp6 mRNA在野生型小鼠的上肠和肝脏中都有表达。Akp3-/-小鼠的肝脏中Akp6 mRNA的表达强度与饲喂高脂肪饮食的野生型小鼠相比没有增强。经鉴定,肝脏中Akp6的PCR产物与肠道中Akp6的核苷酸序列相同。这是关于小鼠肝脏中IAP mRNA表达的第一篇报道,但需要进一步的研究来确定肠型ALP的这种异位表达是否与任何独特的功能有关。
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Expression of intestinal-type alkaline phosphatase mRNA in liver of Akp3 knockout mice
Previously, we discovered that the rat liver showed enhanced expression of intestinal-type alkaline phosphatase (ALP) on high fat-feeding. Here we tested the hypothesis that intestinal-type ALP (Akp3 or Akp6) might also be regulated in the liver of Akp3-/- mice upon high fat-feeding. C57Bl/6J male Akp3-/-, Akp3+/-, or wild-type mice were fed a normal control or high-fat diet and the effects on intestinal-type ALP mRNA in liver were investigated. We found that Akp3 mRNA is only expressed in the intestine and not in the liver, while Akp6 mRNA is expressed in both the upper intestine and liver of wild-type mice. The intensity of Akp6 mRNA expression in the liver was not enhanced in the Akp3-/- mice compared with the wild-type mice fed on a high-fat diet. The nucleotide sequence of the PCR product of Akp6 from the liver was identified as being the same as that of Akp6 in the intestine. This is the first report concerning IAP mRNA expression in the mouse liver, but further studies will be needed to determine if this ectopic expression of intestinal-type ALP is associated with any unique function.
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