S. Meenatchisundaram, R. Selvakumaran, G. Parameswari, A. Michael
{"title":"鸡卵黄抗膨润土和佐剂包被胭脂鱼毒抗体抗蛇毒潜能的比较","authors":"S. Meenatchisundaram, R. Selvakumaran, G. Parameswari, A. Michael","doi":"10.5580/1244","DOIUrl":null,"url":null,"abstract":"The Chicken egg yolk antibodies generated against Bentonite and Adjuvant (FCA) coated Echis carinatus venom was evaluated for their anti-venom potential. Antibodies are extracted from immunized chicken egg yolk by the method described by Polson et al.(1980) and further purified by DEAE cellulose ion exchange column chromatography, which gave pure (180 - 200KDa) specific antibodies against venom. Inhibition of lethal, edema, procoagulant, and phospholipase A2 activities of Echis carinatus venom was determined. We found that 1.27mg of IgY (generated against FCA coated venom) and 1.33mg of IgY (generated against Bentonite coated venom) was able to completely neutralize the lethal activity (2LD 50) of Echis carinatus venom. Both chicken egg yolk antibodies effectively inhibited procoagulant activity and partially inhibited the Edema forming activity. IgY generated using Adjuvant (FCA) coated venoms showed very high anti-phospholipase A2 activity. In general, the results demonstrated the effectiveness of chicken egg yolk antibodies raised against adjuvant and bentonite coated venoms in neutralizing various pharamacological effects of Echis carinatusvenom.Keywords: Venoms, FCA, Bentonite, Chicken antibodies (IgY), PLA 2","PeriodicalId":22523,"journal":{"name":"The Internet Journal of Pharmacology","volume":"45 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Comparison of Antivenom potential of chicken Egg yolk Antibodies Generated against Bentonite and Adjuvant coated Echis carinatus venom\",\"authors\":\"S. Meenatchisundaram, R. Selvakumaran, G. Parameswari, A. Michael\",\"doi\":\"10.5580/1244\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The Chicken egg yolk antibodies generated against Bentonite and Adjuvant (FCA) coated Echis carinatus venom was evaluated for their anti-venom potential. Antibodies are extracted from immunized chicken egg yolk by the method described by Polson et al.(1980) and further purified by DEAE cellulose ion exchange column chromatography, which gave pure (180 - 200KDa) specific antibodies against venom. Inhibition of lethal, edema, procoagulant, and phospholipase A2 activities of Echis carinatus venom was determined. We found that 1.27mg of IgY (generated against FCA coated venom) and 1.33mg of IgY (generated against Bentonite coated venom) was able to completely neutralize the lethal activity (2LD 50) of Echis carinatus venom. Both chicken egg yolk antibodies effectively inhibited procoagulant activity and partially inhibited the Edema forming activity. IgY generated using Adjuvant (FCA) coated venoms showed very high anti-phospholipase A2 activity. In general, the results demonstrated the effectiveness of chicken egg yolk antibodies raised against adjuvant and bentonite coated venoms in neutralizing various pharamacological effects of Echis carinatusvenom.Keywords: Venoms, FCA, Bentonite, Chicken antibodies (IgY), PLA 2\",\"PeriodicalId\":22523,\"journal\":{\"name\":\"The Internet Journal of Pharmacology\",\"volume\":\"45 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet Journal of Pharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/1244\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet Journal of Pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/1244","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Comparison of Antivenom potential of chicken Egg yolk Antibodies Generated against Bentonite and Adjuvant coated Echis carinatus venom
The Chicken egg yolk antibodies generated against Bentonite and Adjuvant (FCA) coated Echis carinatus venom was evaluated for their anti-venom potential. Antibodies are extracted from immunized chicken egg yolk by the method described by Polson et al.(1980) and further purified by DEAE cellulose ion exchange column chromatography, which gave pure (180 - 200KDa) specific antibodies against venom. Inhibition of lethal, edema, procoagulant, and phospholipase A2 activities of Echis carinatus venom was determined. We found that 1.27mg of IgY (generated against FCA coated venom) and 1.33mg of IgY (generated against Bentonite coated venom) was able to completely neutralize the lethal activity (2LD 50) of Echis carinatus venom. Both chicken egg yolk antibodies effectively inhibited procoagulant activity and partially inhibited the Edema forming activity. IgY generated using Adjuvant (FCA) coated venoms showed very high anti-phospholipase A2 activity. In general, the results demonstrated the effectiveness of chicken egg yolk antibodies raised against adjuvant and bentonite coated venoms in neutralizing various pharamacological effects of Echis carinatusvenom.Keywords: Venoms, FCA, Bentonite, Chicken antibodies (IgY), PLA 2
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
