苏云金芽孢杆菌内生壳聚糖分解系统中三种不同胶体壳聚糖生产壳寡糖的比较

D. R. Olicón-Hernández, P. Vazquez-Landaverde, R. Cruz-Camarillo, L. I. Rojas-Avelizapa
{"title":"苏云金芽孢杆菌内生壳聚糖分解系统中三种不同胶体壳聚糖生产壳寡糖的比较","authors":"D. R. Olicón-Hernández, P. Vazquez-Landaverde, R. Cruz-Camarillo, L. I. Rojas-Avelizapa","doi":"10.1080/10826068.2016.1181086","DOIUrl":null,"url":null,"abstract":"ABSTRACT Bacillus thuringiensis is a nonhuman pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Comparison and exploration of the production of chito-oligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in these molecules. This study shows the production of chito-oligosaccharides from three different sources of colloidal chitosan and conducts a qualitative–quantitative comparison between them, using the extracellular enzyme of B. thuringiensis. We found that in the three substrates, it is possible to get a mixture of chito-oligosaccharides from dimer to hexamer in a concentration range from 0.72 to 8.09 mg · g−1 of original substrate. The best substrate to obtain these molecules was commercial chitosan as it has the highest production yields.","PeriodicalId":20393,"journal":{"name":"Preparative Biochemistry and Biotechnology","volume":"20 1","pages":"116 - 122"},"PeriodicalIF":0.0000,"publicationDate":"2017-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"19","resultStr":"{\"title\":\"Comparison of chito-oligosaccharide production from three different colloidal chitosans using the endochitonsanolytic system of Bacillus thuringiensis\",\"authors\":\"D. R. Olicón-Hernández, P. Vazquez-Landaverde, R. Cruz-Camarillo, L. I. Rojas-Avelizapa\",\"doi\":\"10.1080/10826068.2016.1181086\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT Bacillus thuringiensis is a nonhuman pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Comparison and exploration of the production of chito-oligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in these molecules. This study shows the production of chito-oligosaccharides from three different sources of colloidal chitosan and conducts a qualitative–quantitative comparison between them, using the extracellular enzyme of B. thuringiensis. We found that in the three substrates, it is possible to get a mixture of chito-oligosaccharides from dimer to hexamer in a concentration range from 0.72 to 8.09 mg · g−1 of original substrate. The best substrate to obtain these molecules was commercial chitosan as it has the highest production yields.\",\"PeriodicalId\":20393,\"journal\":{\"name\":\"Preparative Biochemistry and Biotechnology\",\"volume\":\"20 1\",\"pages\":\"116 - 122\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-02-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"19\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Preparative Biochemistry and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/10826068.2016.1181086\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Preparative Biochemistry and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10826068.2016.1181086","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 19

摘要

苏云金芽孢杆菌是一种非人类病原菌,被用作真菌和昆虫的生物防治剂。由于其与环境的相互作用,它具有几种能够降解几丁质和壳聚糖的细胞外酶,这两种最重要的聚合物因其在许多领域的应用而得到广泛应用。然而,近年来,人们已经观察到壳聚糖酶降解产生的低聚糖对人体健康有重要的益处。对不同来源壳聚糖制备壳寡糖的工艺进行比较和探索,将有助于改进工艺参数,扩大生物技术的应用范围。本研究展示了三种不同来源的胶体壳聚糖生产壳寡糖,并利用苏云金芽孢杆菌胞外酶对它们进行了定性定量比较。我们发现,在这三种底物中,可以得到从二聚体到六聚体的壳寡糖混合物,其浓度范围为0.72 ~ 8.09 mg·g−1。商品壳聚糖是制备这些分子的最佳底物,产率最高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Comparison of chito-oligosaccharide production from three different colloidal chitosans using the endochitonsanolytic system of Bacillus thuringiensis
ABSTRACT Bacillus thuringiensis is a nonhuman pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Comparison and exploration of the production of chito-oligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in these molecules. This study shows the production of chito-oligosaccharides from three different sources of colloidal chitosan and conducts a qualitative–quantitative comparison between them, using the extracellular enzyme of B. thuringiensis. We found that in the three substrates, it is possible to get a mixture of chito-oligosaccharides from dimer to hexamer in a concentration range from 0.72 to 8.09 mg · g−1 of original substrate. The best substrate to obtain these molecules was commercial chitosan as it has the highest production yields.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Effect of pretreatment with organic solvent on enzymatic digestibility of cauliflower wastes Selective removal of closely related clipped protein impurities using poly(ethylenimine)- grafted anion-exchange chromatography resin A colorimetric method for the determination of different functional flavonoids using 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and peroxidase Optimization of propionic acid production in apple pomace extract with Propionibacterium freudenreichii Optimization of fermented Perilla frutescens seeds for enhancement of gamma-aminobutyric acid and bioactive compounds by Lactobacillus casei TISTR 1500
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1