苏云金芽孢杆菌内生壳聚糖分解系统中三种不同胶体壳聚糖生产壳寡糖的比较

D. R. Olicón-Hernández, P. Vazquez-Landaverde, R. Cruz-Camarillo, L. I. Rojas-Avelizapa
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引用次数: 19

摘要

苏云金芽孢杆菌是一种非人类病原菌,被用作真菌和昆虫的生物防治剂。由于其与环境的相互作用,它具有几种能够降解几丁质和壳聚糖的细胞外酶,这两种最重要的聚合物因其在许多领域的应用而得到广泛应用。然而,近年来,人们已经观察到壳聚糖酶降解产生的低聚糖对人体健康有重要的益处。对不同来源壳聚糖制备壳寡糖的工艺进行比较和探索,将有助于改进工艺参数,扩大生物技术的应用范围。本研究展示了三种不同来源的胶体壳聚糖生产壳寡糖,并利用苏云金芽孢杆菌胞外酶对它们进行了定性定量比较。我们发现,在这三种底物中,可以得到从二聚体到六聚体的壳寡糖混合物,其浓度范围为0.72 ~ 8.09 mg·g−1。商品壳聚糖是制备这些分子的最佳底物,产率最高。
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Comparison of chito-oligosaccharide production from three different colloidal chitosans using the endochitonsanolytic system of Bacillus thuringiensis
ABSTRACT Bacillus thuringiensis is a nonhuman pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Comparison and exploration of the production of chito-oligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in these molecules. This study shows the production of chito-oligosaccharides from three different sources of colloidal chitosan and conducts a qualitative–quantitative comparison between them, using the extracellular enzyme of B. thuringiensis. We found that in the three substrates, it is possible to get a mixture of chito-oligosaccharides from dimer to hexamer in a concentration range from 0.72 to 8.09 mg · g−1 of original substrate. The best substrate to obtain these molecules was commercial chitosan as it has the highest production yields.
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