超声波提取葫芦叶提取物多酚含量及抗氧化活性的研究

Lamija Kolarević, E. Horozić, Z. Ademovic, Ermina Cilović Kozarević
{"title":"超声波提取葫芦叶提取物多酚含量及抗氧化活性的研究","authors":"Lamija Kolarević, E. Horozić, Z. Ademovic, Ermina Cilović Kozarević","doi":"10.9734/irjpac/2023/v24i4815","DOIUrl":null,"url":null,"abstract":"In this research, the antioxidant activity and its correlation with the polyphenolic content in pumpkin leaf extracts (Cucurbita pepo L.) were examined. Dried and pulverized pumpkin leaves were used as extraction material. Various solvents (water, methanol, ethanol, acetone) and their mixtures, in a ratio of 50:50 (v/v) (water: methanol, water: ethanol, water: acetone) were used for extraction. The solid-to-solvent ratio was 1:10. The influence of solvents on phenolic extraction, as well as the effect of ultrasonic extraction was investigated. The samples were subjected to ultrasound for 15 minutes. The total phenolic content was determined by the Folin-Ciocalteu method and the antioxidant activity of the extracts by FRAP and DPPH methods. The obtained results indicate the importance of choosing an adequate extraction solvent for phenolic isolation from plant material. Mixtures of organic solvents and water, especially a mixture of water and acetone, are the most suitable for the extraction of phenolic compounds. At the same time, a positive correlation was established between the content of total phenols and the antioxidant activity of the extracts. This suggests that phenols contribute significantly to the antioxidant properties of pumpkin leaves. The results showed the potential medicinal properties of pumpkin leaves but further studies are needed to identify, characterize and isolate different bioactive components, which could be used as a basis for obtaining new drugs for the treatment of various diseases.","PeriodicalId":14371,"journal":{"name":"International Research Journal of Pure and Applied Chemistry","volume":"78 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of Polyphenol Content and Antioxidative Activity of Cucurbita pepo L. Leaf Extracts Obtained by Ultrasonic Extraction\",\"authors\":\"Lamija Kolarević, E. Horozić, Z. Ademovic, Ermina Cilović Kozarević\",\"doi\":\"10.9734/irjpac/2023/v24i4815\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this research, the antioxidant activity and its correlation with the polyphenolic content in pumpkin leaf extracts (Cucurbita pepo L.) were examined. Dried and pulverized pumpkin leaves were used as extraction material. Various solvents (water, methanol, ethanol, acetone) and their mixtures, in a ratio of 50:50 (v/v) (water: methanol, water: ethanol, water: acetone) were used for extraction. The solid-to-solvent ratio was 1:10. The influence of solvents on phenolic extraction, as well as the effect of ultrasonic extraction was investigated. The samples were subjected to ultrasound for 15 minutes. The total phenolic content was determined by the Folin-Ciocalteu method and the antioxidant activity of the extracts by FRAP and DPPH methods. The obtained results indicate the importance of choosing an adequate extraction solvent for phenolic isolation from plant material. Mixtures of organic solvents and water, especially a mixture of water and acetone, are the most suitable for the extraction of phenolic compounds. At the same time, a positive correlation was established between the content of total phenols and the antioxidant activity of the extracts. This suggests that phenols contribute significantly to the antioxidant properties of pumpkin leaves. The results showed the potential medicinal properties of pumpkin leaves but further studies are needed to identify, characterize and isolate different bioactive components, which could be used as a basis for obtaining new drugs for the treatment of various diseases.\",\"PeriodicalId\":14371,\"journal\":{\"name\":\"International Research Journal of Pure and Applied Chemistry\",\"volume\":\"78 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-07-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Research Journal of Pure and Applied Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/irjpac/2023/v24i4815\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Research Journal of Pure and Applied Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/irjpac/2023/v24i4815","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

研究了南瓜叶提取物的抗氧化活性及其与多酚含量的关系。以干燥粉碎的南瓜叶为提取原料。采用各种溶剂(水、甲醇、乙醇、丙酮)及其混合物,以50:50 (v/v)的比例(水:甲醇、水:乙醇、水:丙酮)进行提取。固液比为1:10。考察了溶剂对苯酚提取的影响,以及超声波提取的效果。样品接受超声检查15分钟。用Folin-Ciocalteu法测定总酚含量,用FRAP法和DPPH法测定提取物的抗氧化活性。实验结果表明,选择合适的萃取溶剂对植物中酚类物质的分离具有重要意义。有机溶剂与水的混合物,特别是水与丙酮的混合物,最适合提取酚类化合物。同时,总酚含量与提取物的抗氧化活性呈正相关。这表明酚类物质对南瓜叶的抗氧化性能有重要作用。结果表明,南瓜叶具有潜在的药用价值,但需要进一步的研究来鉴定、表征和分离不同的生物活性成分,为获得治疗各种疾病的新药提供基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Investigation of Polyphenol Content and Antioxidative Activity of Cucurbita pepo L. Leaf Extracts Obtained by Ultrasonic Extraction
In this research, the antioxidant activity and its correlation with the polyphenolic content in pumpkin leaf extracts (Cucurbita pepo L.) were examined. Dried and pulverized pumpkin leaves were used as extraction material. Various solvents (water, methanol, ethanol, acetone) and their mixtures, in a ratio of 50:50 (v/v) (water: methanol, water: ethanol, water: acetone) were used for extraction. The solid-to-solvent ratio was 1:10. The influence of solvents on phenolic extraction, as well as the effect of ultrasonic extraction was investigated. The samples were subjected to ultrasound for 15 minutes. The total phenolic content was determined by the Folin-Ciocalteu method and the antioxidant activity of the extracts by FRAP and DPPH methods. The obtained results indicate the importance of choosing an adequate extraction solvent for phenolic isolation from plant material. Mixtures of organic solvents and water, especially a mixture of water and acetone, are the most suitable for the extraction of phenolic compounds. At the same time, a positive correlation was established between the content of total phenols and the antioxidant activity of the extracts. This suggests that phenols contribute significantly to the antioxidant properties of pumpkin leaves. The results showed the potential medicinal properties of pumpkin leaves but further studies are needed to identify, characterize and isolate different bioactive components, which could be used as a basis for obtaining new drugs for the treatment of various diseases.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Kinetics and Equilibrium Studies of the Detoxification of Aqueous Solutions of Phenolic Derivatives using Activated Carbon Anti-Diabetic Activities of the Extracts from Euphorbia hirta L. (Euphorbiaceae) Specie found in Burkina Faso Using α-Glucosidase Inhibitor Syntheses and X-ray Structure of N-(Benzothiazol-2-yl)-3-chlorobenzamide Copper Adsorption by a Clay from Central Ivory Coast: Analysis by Differential Pulse Anodic Stripping Voltammetry Isolation and Characterization of Glucocorticoid Steriod from the Leaf of Rauvolfia vomitoria
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1