A two-pinhole technique to determine distribution profiles of relative elemental growth rates in the growth zone of grass leaves.

A new modified pricking technique, a two-pinhole method, was designed to determine the spatial distribution of leaf elongation of grasses. This new technique makes it possible to obtain the distribution profiles of relative elemental growth rates in the growth zone, to evaluate the effect of pricking on the distribution profile of leaf elongation in the growth zone and to decrease the reduction in the elongation rate of grass leaves due to pricking.