Genetic Confirmation of Mungbean Genotypes (Vigna Radiata L. Wilczek) Using Molecular Markers

Molecular characterization is helpful in understanding the phylogenetic relationship among various germplasm to reveal the genetic diversity among the used parental genotypes. Among several efficient methods for revealing genetic variability within and among plant populations, one of the most widely applied method is marker analysis. RAPD and ISSR, markers are commonly used because they are quick, simple and environment non-sensitive enabling genetic diversity analysis in several types of plant material like natural populations, population in breeding programmes. Evaluation of genetic diversity would promote the efficient use of genetic variations, effective conservation and purity of the genotype to be determined as well as utilization of germplasm in crop improvement. The RAPD and ISSR data were evaluated to obtain a combined similarity matrix. The similarity coefficient values lay between 0.46–0.68. The RAPD and ISSR cluster tree analysis showed that the eight genotypes could be divided into 4 clusters. The genotype BM 4 was grouped in separate VI cluster. However, PDM 139 was grouped on cluster IIA. In the light of RAPD and ISSR study the parents of the cross BM 4 x PDM 139 were also noticed for their genetic diversity, having 53% dissimilarity and grouped into the separate clusters.