{"title":"DP受体激动剂治疗后巨噬细胞自然免疫相关功能改变的同时测定","authors":"Kiyoshi Daito, Y. Azuma, M. Daito, K. Ohura","doi":"10.2330/JORALBIOSCI1965.44.522","DOIUrl":null,"url":null,"abstract":"Prostaglandin D2 (PGD2) acts via the adenyl cyclase-coupled receptor for PGD2 (DP receptor). Here we present evidence that BW245C, a DP receptor agonist, modulates macrophage functions related to natunal immunity. BW245C inhibited macrophage chemotaxis at concentrations of 0.1 to 10μM and phagocytosis of Escherichia coli by macrophages at a concentration of 10μM. In addition, BW245C inhibited the production of superoxide anions by PMA-stimulated macrophages at concentrations of 0.1 to 10μM and nitrite production by LPS-stimulated macrophages at a concentration of 10μM. In contrast, BW245C potentiated the production of TNF-α, a pro-inflammatory cytokine, by LPS-stimulated macrophages at concentrations of 1 to 10μM. These results suggest that PGD2 may modulate macrophage functions related to natural immunity via the DP receptor.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"24 1","pages":"522-529"},"PeriodicalIF":0.0000,"publicationDate":"2002-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Simultaneous Determination of Alteration of a Variety of Macrophage Functions Related to Natural Immunity Following Treatment with a DP Receptor Agonist\",\"authors\":\"Kiyoshi Daito, Y. Azuma, M. Daito, K. Ohura\",\"doi\":\"10.2330/JORALBIOSCI1965.44.522\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Prostaglandin D2 (PGD2) acts via the adenyl cyclase-coupled receptor for PGD2 (DP receptor). Here we present evidence that BW245C, a DP receptor agonist, modulates macrophage functions related to natunal immunity. BW245C inhibited macrophage chemotaxis at concentrations of 0.1 to 10μM and phagocytosis of Escherichia coli by macrophages at a concentration of 10μM. In addition, BW245C inhibited the production of superoxide anions by PMA-stimulated macrophages at concentrations of 0.1 to 10μM and nitrite production by LPS-stimulated macrophages at a concentration of 10μM. In contrast, BW245C potentiated the production of TNF-α, a pro-inflammatory cytokine, by LPS-stimulated macrophages at concentrations of 1 to 10μM. These results suggest that PGD2 may modulate macrophage functions related to natural immunity via the DP receptor.\",\"PeriodicalId\":14631,\"journal\":{\"name\":\"Japanese Journal of Oral Biology\",\"volume\":\"24 1\",\"pages\":\"522-529\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese Journal of Oral Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2330/JORALBIOSCI1965.44.522\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Oral Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2330/JORALBIOSCI1965.44.522","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Simultaneous Determination of Alteration of a Variety of Macrophage Functions Related to Natural Immunity Following Treatment with a DP Receptor Agonist
Prostaglandin D2 (PGD2) acts via the adenyl cyclase-coupled receptor for PGD2 (DP receptor). Here we present evidence that BW245C, a DP receptor agonist, modulates macrophage functions related to natunal immunity. BW245C inhibited macrophage chemotaxis at concentrations of 0.1 to 10μM and phagocytosis of Escherichia coli by macrophages at a concentration of 10μM. In addition, BW245C inhibited the production of superoxide anions by PMA-stimulated macrophages at concentrations of 0.1 to 10μM and nitrite production by LPS-stimulated macrophages at a concentration of 10μM. In contrast, BW245C potentiated the production of TNF-α, a pro-inflammatory cytokine, by LPS-stimulated macrophages at concentrations of 1 to 10μM. These results suggest that PGD2 may modulate macrophage functions related to natural immunity via the DP receptor.
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