An analytical tooth model based on SPR chips coated with hydroxyapatite used for investigation of the acquired dental pellicle

One of the main targets in modern dentistry is the prevention of caries. Not only daily nutrition plays an important role, but also the so called “pellicle” on the outer surfaced of teeth, which is mainly formed by saliva proteins. Moreover, numerous bacteria are part of this pellicle, which might cause caries by acidic metabolites. In the here presented study, a method for electrophoretic deposition (EPD) of hydroxyapatite (HAP) nanoparticles on gold surfaces, suitable for Surface Plasmon Resonance (SPR) measurements has been developed. An “artificial tooth” has been created and loaded by natural saliva in different concentrations (to form a natural pellicle), while the influence of an environment with different pH values has been studied. It could be demonstrated that even slight acid solutions damage the pellicle significantly within seconds, exposing the HAP to acidic degradation, which would lead to caries in the human oral cavities. This model allows to study pellicle formation as well as degradation in real time. As a practical example, the influence of beverages on the pellicle could be demonstrated.This article is protected by copyright. All rights reserved.