杜仲提取物通过抑制内质网应激和氧化应激减轻大鼠脊髓损伤

H. Cheng, Haoyu Dong, Jie Wei, Guopeng You
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摘要

脊髓损伤(SCI)是世界范围内严重致残的主要原因,可导致神经元死亡、神经元变性和脊髓功能改变。杜仲提取物(Eucommia ulmoides extract, EUE)是由多种环烯醚萜类和酚类化合物组成,在多种病理中具有抗氧化和抗炎作用。本研究旨在探讨欧盟对脊髓损伤的影响及其机制。雄性成年Sprague-Dawley大鼠(250 ~ 280 g)在体内模拟脊髓损伤。Western blot和ELISA试剂盒检测细胞凋亡、氧化应激和内质网应激相关蛋白的表达。采用TUNEL法测定脊髓标本中神经元的凋亡率。最后,我们的数据表明,EUE通过抑制丝裂原活化蛋白激酶(MAPK)途径抑制sci诱导的细胞凋亡、氧化应激和内质网应激。我们的数据表明EUE是脊髓损伤的潜在治疗靶点。
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Eucommia ulmoides extract alleviated spinal cord injury in rats by inhibiting endoplasmic reticulum stress and oxidative stress
Spinal cord injury (SCI) is the major cause of severe disability worldwide, which leads to neuron death, neuronal degeneration, and functional changes in the spinal cord. Eucommia ulmoides extract (EUE) is composed of mul-tiple iridoids and phenols and possesses anti-oxidative and anti-inflammatory effects in various pathologies. This study aims to evaluate the effects of EUE on SCI and the underlying mechanisms. Male adult Sprague-Dawley rats (250–280 g) were applied to mimic SCI in vivo. Western blot and ELISA kits assessed the expressions of apoptosis, oxidative stress, and endoplasmic reticulum stress (ER stress)-relevant proteins. The apoptosis rate of neurons in spinal cord specimens was measured by TUNEL assay. Finally, our data indicated that EUE inhibited SCI-induced apoptosis, oxidative stress, and ER stress through the suppression of mitogen-activated protein kinase (MAPK) pathway. Our data manifest EUE as a potential therapeutic target in SCI.
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