苦杏仁提取物作为家禽抗微生物饲料添加剂的潜力

Matilda E. Weniafere, Ada Yaabari Nkpegamee, Ruth Ofongo, E. Ohimain
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摘要

由于抗生素耐药性的问题,合成抗生素在动物养殖中的使用受到限制,这刺激了对替代品的开发研究。本试验旨在研究苦叶水乙醇提取物对沙门氏菌、志贺氏菌、葡萄球菌、大肠杆菌和铜绿假单胞菌的体外抗菌活性。采用Kirby-Bauer纸片扩散法进行药敏试验。得到的结果表明,没有一种被测试的生物对20%的水提取物敏感。水提取物的灵敏度从40%提高到100%。葡萄球菌的最高抑制区(ZOI)为17.00±1.00 mm,铜绿假单胞菌的最高抑制区(ZOI)为16.33±1.15 mm,均为100%。当乙醇提取物浓度为300g /ml时,苦叶乙醇提取物对葡萄球菌的ZOI最高(27.67±2.52 mm),对大肠杆菌的ZOI最高(25.00±2.00 mm)。因此,我们得出结论,苦叶可以被认为是家禽合成抗生素生长促进剂的潜在替代品。
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Potentials of Veronica amygdalina extracts as an alternative anti-microbial feed additive in poultry
Due to the problem of antibiotic resistance, the use of synthetic antibiotics has been restricted in animal farming, which has spurred research into the development of alternatives. This study was designed to assess the in vitro antibiotic activity of the water and ethanolic extract of bitter leaf against Salmonella sp, Shigella sp, Staphylococcus sp, Escherichia coli and Pseudomonas aeruginosa. Antimicrobial susceptibility test was done using Kirby-Bauer disc diffusion method. Results obtained showed that none of the organism tested was sensitive at 20% of the water extract. Sensitivity increased from 40% to 100% of the water extract. The highest zone of inhibition (ZOI) of 17.00±1.00 mm was obtained for Staphylococcus sp, followed by 16.33±1.15 mm for P. aeruginosa both at 100%. The highest ZOI for the ethanolic extract of bitter leaf (27.67±2.52 mm) was recorded for Staphylococcus sp followed by 25.00±2.00   mm for E. coli both at ethanol extract concentration of 300g /ml. We therefore conclude that bitter leaf can be considered as potential alternative to synthetic antibiotic growth promoters in poultry.
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