随机扩增多态性DNA (RAPD)技术在红潮浮游植物查通菌种类鉴定中的应用

Eiko Murayama-Kayano , Sadaaki Yoshimatsu , Toshiaki Kayano , Takeshi Nishio , Hiroshi Ueda , Teruyuki Nagamune
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引用次数: 8

摘要

采用随机扩增多态性DNA (RAPD)技术进行品系和种间鉴定。采用4种任意引物对4种查顿菌属赤潮藻7株的dna进行了检测。其中两个引物显示了物种依赖的特征,而另外两个引物则确定了菌株之间的差异。结果表明,RAPD技术可用于区分种或品系间的多态性。
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Application of the random amplified polymorphic DNA (RAPD) technique to distinguishing species of the red tide phytoplankton Chattonella (Raphydophyceae)

The random amplified polymorphic DNA (RAPD) technique was applied to strain and species identification. Four different arbitrary primers were tested with DNAs from seven strains of four species of red tide algae of the genus Chattonella. Two of the primers showed species-dependent profiles, while the other two identified differences between strains. The results indicate that the RAPD technique is useful for distinguishing polymorphisms among species or strains.

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