利用廉价的叶绿素荧光仪探索光合作用和植物胁迫

Stephen Cessna, Barbara Demmig-Adams, William W. Adams III
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引用次数: 33

摘要

掌握光合作用的概念对学习植物生理学及其应用至关重要,但似乎是生物学中更具挑战性的概念之一。毫无疑问,由于植物在不同环境中改变光合作用的复杂性,这一教学挑战变得更加复杂。在这里,我们建议在本科植物生理学课堂上使用叶绿素荧光仪,作为加强光合作用及其适应变化环境的概念学习的一种手段。我们还概述了光合适应变化条件的研究现状,回顾了使用小型廉价荧光仪进行良好的Fv/Fm测量的方法考虑,并建议在本科植物生理学课程中使用荧光仪进行光合作用和光适应的开放探究活动。
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Exploring Photosynthesis and Plant Stress Using Inexpensive Chlorophyll Fluorometers

Mastering the concept of photosynthesis is of critical importance to learning plant physiology and its applications, but seems to be one of the more challenging concepts in biology. This teaching challenge is no doubt compounded by the complexity by which plants alter photosynthesis in different environments. Here we suggest the use of chlorophyll fluorometers in the undergraduate plant physiology classroom as one means to enhance conceptual learning of photosynthesis and its acclimation to changing environments. We also provide an overview of current research in photosynthetic acclimation to changing conditions, review the methodological considerations of making good Fv/Fm measurements with small inexpensive fluorometers, and suggest an open-inquiry activity for teaching concepts of photosynthesis and photo-acclimation with fluorometers in an undergraduate plant physiology course.

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