水和蔬菜样品中百草枯全步合一检测试剂盒

Analytica Pub Date : 2022-02-23 DOI:10.3390/analytica3010007
Chanakarn Sangsum, P. Saetear
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引用次数: 1

摘要

本工作首次提出了一种用于测定天然水、蔬菜和农业样品中百草枯的全步骤一体测试试剂盒。手持式光度计结合磁力搅拌器完成提取、混合和检测步骤。百草枯在碱性条件下与二亚硫酸钠还原产生蓝色自由基离子。研究了二亚硫酸钠粉末对试剂稳定性持续时间的增强作用,将二亚硫酸钠粉末直接加入到样品溶液中,与二亚硫酸钠溶液形式相比,灵敏度差异不显著。该试剂盒在0.5 ~ 10 mg L−1的线性定标范围内表现良好,具有较高的测定系数(r2 = 0.9947)。手持式光度计定量下限为0.50 mg L−1 (LLOQ = 3SD /斜率截距)。肉眼检出限(LOD)为0.30 mg L−1。回收率研究在101-115%范围内可接受。日内(n = 3)和日内(n = 3)精度均小于1%。在95%置信区间的显著性检验基础上,开发的检测试剂盒的定量结果与高效液相色谱法(HPLC)的定量结果吻合良好。据我们所知,这是第一个展示在线提取蔬菜的测试试剂盒,适用于现场分析的报告。基于比尔-朗伯定律的单点校准也演示了百草枯的测量。在以5.00 mg L−1百草枯标称标准溶液进行检测时,读数浓度为5.09±0.03 mg L−1百草枯(n = 20), K值为0.0967(接近多点校准斜率)。这项研究对农药和除草剂的分析直接有利于农产品和人口的健康。
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All-Step-in-One Test Kit for Paraquat Detection in Water and Vegetable Samples
This work presents the first development of an all-steps-in-one test kit for the determination of paraquat in natural water, and vegetable and agricultural samples. A handheld photometer incorporated with a magnetic stirrer was used to complete the steps of extraction, mixing, and detection. Paraquat produces a blue free radical ion via a reduction with sodium dithionite in alkaline conditions. Sodium dithionite powder was investigated for the enhancement of reagent stability duration, which was added directly into sample solution that showed insignificant difference in sensitivity as compared with that of the solution format of sodium dithionite. The developed test kit showed good performance with the linear calibration of 0.5 to 10 mg L−1 with a high coefficient of determination (r2 = 0.9947). The lower limit of quantitation (LLOQ = 3SD of intercept per slope) carried out from the method using the handheld photometer was 0.50 mg L−1. The limit of detection (LOD) by naked eye was 0.30 mg L−1. The recovery study was acceptable in the range of 101–115%. Intraday (n = 3) and interday (n = 3) precision was less than 1%. On the basis of the significance test at the 95% confidence interval, quantitative results of the developed test kit agreed well with those from high-performance liquid chromatography (HPLC). To the best of our knowledge, this is the first report demonstrating an online extraction for vegetables incorporated into a test kit, applicable for on-site analysis. Single-point calibration based on the Beer–Lambert law also demonstrated the measurement of paraquat. In testing with a nominal standard solution of 5.00 mg L−1 paraquat, the reading concentration was 5.09 ± 0.03 mg L−1 paraquat (n = 20) with a K value of 0.0967 (close to the slope of multipoint calibration). This research is a direct benefit to agricultural products and the health of a population for the analysis of pesticides and herbicides.
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