Mihály István, Bod Réka-Barbara, O. Károly, Berki Ádám-József, Szilágyi Tibor
{"title":"脑深部刺激对慢性癫痫模型高频振荡的影响","authors":"Mihály István, Bod Réka-Barbara, O. Károly, Berki Ádám-József, Szilágyi Tibor","doi":"10.2478/orvtudert-2020-0014","DOIUrl":null,"url":null,"abstract":"Abstract Temporal lobe epilepsy (TLE) is a severe neurological disease which is often pharmacoresistant. Deep brain stimulation (DBS) is a novel method for treating epilepsy; however, its mechanism of action is not fully understood. We aimed to study the effect of amygdala DBS in the pilocarpine model of TLE. Status epilepticus was induced by pilocarpine in male Wistar rats, and spontaneous seizures occurred after a latency period. A stimulating electrode was inserted into the left basolateral amygdala and two recording electrodes into the left and right hippocampus. A stimulus package consisted of 0.1 ms-long biphasic pulses applied regularly at 4 Hz for 50 seconds. This package was repeated four times a day, with 5-minute pauses, for 10 days. We also used an age-matched healthy control group of stimulated animals and another one of sham-operated rats. From the hippocampal local field potentials high frequency oscillations (HFOs) were analyzed as these are promising epilepsy biomarkers. HFOs are short oscillatory events between 80-600 Hz which were detected offline using an open-source application of MATLAB, the RIPPLELAB system. We found that the HFO rate was significantly higher in pilocarpine-treated rats compared to the control groups (0.41 ± 0.14 HFO/min vs. 0.006 ± 0.003 in the stimulated control group and no HFO in the sham-operated group). In the pilocarpine group an instantaneous decrease in HFO rate was observed while the stimulation was on (0.44 ± 0.15 HFO/min vs 0.07 ± 0.03 HFO/min, p=0.017). The effect was short-lived because the frequency of HFOs did not change significantly in the time windows between stimulus packages or during the ten-day stimulation period. The difference of HFO rates between epileptic and control groups could be used in the electrographic assessment of epilepsy. The decreased frequency of HFOs during stimulation may be useful to study the efficacy of DBS.","PeriodicalId":9334,"journal":{"name":"Bulletin of Medical Sciences","volume":"57 1","pages":"63 - 70"},"PeriodicalIF":0.0000,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Effect of Deep Brain Stimulation on High Frequency Oscillations in a Chronic Epilepsy Model\",\"authors\":\"Mihály István, Bod Réka-Barbara, O. Károly, Berki Ádám-József, Szilágyi Tibor\",\"doi\":\"10.2478/orvtudert-2020-0014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Temporal lobe epilepsy (TLE) is a severe neurological disease which is often pharmacoresistant. Deep brain stimulation (DBS) is a novel method for treating epilepsy; however, its mechanism of action is not fully understood. We aimed to study the effect of amygdala DBS in the pilocarpine model of TLE. Status epilepticus was induced by pilocarpine in male Wistar rats, and spontaneous seizures occurred after a latency period. A stimulating electrode was inserted into the left basolateral amygdala and two recording electrodes into the left and right hippocampus. A stimulus package consisted of 0.1 ms-long biphasic pulses applied regularly at 4 Hz for 50 seconds. This package was repeated four times a day, with 5-minute pauses, for 10 days. We also used an age-matched healthy control group of stimulated animals and another one of sham-operated rats. From the hippocampal local field potentials high frequency oscillations (HFOs) were analyzed as these are promising epilepsy biomarkers. HFOs are short oscillatory events between 80-600 Hz which were detected offline using an open-source application of MATLAB, the RIPPLELAB system. We found that the HFO rate was significantly higher in pilocarpine-treated rats compared to the control groups (0.41 ± 0.14 HFO/min vs. 0.006 ± 0.003 in the stimulated control group and no HFO in the sham-operated group). In the pilocarpine group an instantaneous decrease in HFO rate was observed while the stimulation was on (0.44 ± 0.15 HFO/min vs 0.07 ± 0.03 HFO/min, p=0.017). The effect was short-lived because the frequency of HFOs did not change significantly in the time windows between stimulus packages or during the ten-day stimulation period. The difference of HFO rates between epileptic and control groups could be used in the electrographic assessment of epilepsy. The decreased frequency of HFOs during stimulation may be useful to study the efficacy of DBS.\",\"PeriodicalId\":9334,\"journal\":{\"name\":\"Bulletin of Medical Sciences\",\"volume\":\"57 1\",\"pages\":\"63 - 70\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Medical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/orvtudert-2020-0014\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Medical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/orvtudert-2020-0014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
颞叶癫痫(TLE)是一种严重的神经系统疾病,通常具有耐药性。脑深部电刺激(DBS)是一种治疗癫痫的新方法;然而,其作用机制尚不完全清楚。我们的目的是研究杏仁核DBS在匹罗卡平TLE模型中的作用。匹罗卡品诱导雄性Wistar大鼠癫痫持续状态,并在潜伏期后发生自发性癫痫发作。一个刺激电极插入左基底外侧杏仁核,两个记录电极分别插入左海马和右海马。刺激方案包括0.1毫秒长的双相脉冲,以4赫兹的频率定期施加50秒。每天重复四次,每隔5分钟暂停一次,持续10天。我们还使用了一组年龄匹配的健康受刺激动物和另一组假手术大鼠作为对照组。海马局部场电位高频振荡(HFOs)是一种有前景的癫痫生物标志物。hfo是80- 600hz之间的短振荡事件,使用MATLAB的开源应用程序RIPPLELAB系统离线检测。我们发现,匹罗卡品处理大鼠的HFO率明显高于对照组(刺激组为0.41±0.14 HFO/min,对照组为0.006±0.003 HFO/min,假手术组无HFO)。匹罗卡平组在刺激时HFO率瞬间下降(0.44±0.15 HFO/min vs 0.07±0.03 HFO/min, p=0.017)。这种影响是短暂的,因为在刺激方案之间的时间窗口或在10天的刺激期间,hfo的频率没有显著变化。癫痫组与对照组HFO率的差异可用于癫痫的电图评价。刺激过程中HFOs频率的降低可能有助于研究DBS的疗效。
The Effect of Deep Brain Stimulation on High Frequency Oscillations in a Chronic Epilepsy Model
Abstract Temporal lobe epilepsy (TLE) is a severe neurological disease which is often pharmacoresistant. Deep brain stimulation (DBS) is a novel method for treating epilepsy; however, its mechanism of action is not fully understood. We aimed to study the effect of amygdala DBS in the pilocarpine model of TLE. Status epilepticus was induced by pilocarpine in male Wistar rats, and spontaneous seizures occurred after a latency period. A stimulating electrode was inserted into the left basolateral amygdala and two recording electrodes into the left and right hippocampus. A stimulus package consisted of 0.1 ms-long biphasic pulses applied regularly at 4 Hz for 50 seconds. This package was repeated four times a day, with 5-minute pauses, for 10 days. We also used an age-matched healthy control group of stimulated animals and another one of sham-operated rats. From the hippocampal local field potentials high frequency oscillations (HFOs) were analyzed as these are promising epilepsy biomarkers. HFOs are short oscillatory events between 80-600 Hz which were detected offline using an open-source application of MATLAB, the RIPPLELAB system. We found that the HFO rate was significantly higher in pilocarpine-treated rats compared to the control groups (0.41 ± 0.14 HFO/min vs. 0.006 ± 0.003 in the stimulated control group and no HFO in the sham-operated group). In the pilocarpine group an instantaneous decrease in HFO rate was observed while the stimulation was on (0.44 ± 0.15 HFO/min vs 0.07 ± 0.03 HFO/min, p=0.017). The effect was short-lived because the frequency of HFOs did not change significantly in the time windows between stimulus packages or during the ten-day stimulation period. The difference of HFO rates between epileptic and control groups could be used in the electrographic assessment of epilepsy. The decreased frequency of HFOs during stimulation may be useful to study the efficacy of DBS.