新型杀虫剂防治枣椰果害虫Ephestia cautella (Walker)的全基因组DNA突变及生化效应

F. A. Ahmed, M. Rashed, Hala Abou-Yousf, E. Abdel-Rahim, S. Mahdi, G. Osman, M. Atia
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引用次数: 1

摘要

枣椰果在成熟的各个阶段都有不同的害虫。杏仁蛾(Ephestia cautella)是一种破坏储存产品的昆虫,如枣、小麦粉和坚果。本研究评估了对人体安全、对环境影响相对较小的新型杀虫剂(Spinosyns、Diamide、Pyridalyl和印楝素)的使用情况。重点评价了5种杀虫剂(Tracer、Radiant、Coragen、Pleo和Achook)的毒理学和生化效应,并筛选了这些杀虫剂引起的全基因组DNA突变。采用生物测定法测定了各杀虫剂的LC50值,结果表明,Coragen的LC50值为0.49 ppm,是最有效的杀虫剂,其次是Radiant和Achook (LC50值分别为1.51和1.73 ppm)。此外,还研究了这些杀虫剂对重要酶的影响。这项研究的数据显示,辐射治疗显示出AST、ACP、ALP和GST活性的刺激,以及AChE、ALT和蛋白酶活性的抑制。胶原蛋白处理后,AChE、GST、ALP、ACP、ALT和蛋白酶活性均明显升高。最后,利用RAPD-PCR方法分析了杀虫剂引起的dna水平的变异,结果表明辐射杀虫剂是一种基因毒性杀虫剂,在基因组- dna水平上引起了很大的变化。另一方面,胶原蛋白对昆虫DNA的变异作用最小。
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Genome-wide DNA Mutability and Biochemical Effects of Novel Insecticides in the Control of Date Palm Fruit Pest Ephestia cautella (Walker)
Different pests attack date palm fruits during all the stages of maturity. Almond moth (Ephestia cautella) is a destructive insect of stored products, such as dates, wheat flour, and nuts. In the present study, the usage of novel groups of insecticides (Spinosyns, Diamide, Pyridalyl, and Azadirachtin) that are safe for humans and exert a relatively lower effect on the environment was assessed. In particular, the toxicological and biochemical impacts of five insecticides (Tracer, Radiant, Coragen, Pleo, and Achook) on the control of E. Cautella were evaluated, and the genome-wide DNA mutability caused by these insecticides was screened. A bioassay was performed in order to determine the LC50value for each insecticide, the results of which revealed that Coragen with an LC50 value of 0.49 ppm was the most potent insecticide, followed by Radiant and Achook (with LC50 values of 1.51 and 1.73 ppm, respectively). In addition, the effect of these insecticides on vital enzymes was investigated. The data from this investigation revealed that the treatment with Radiant demonstrated stimulation in AST, ACP, ALP, and GST activity, and inhibition in AChE, ALT, and protease activity. The insects treated with Coragen exhibited an elevation in the activities of AChE, GST, ALP, ACP, ALT, and protease. Finally, DNA-level mutability caused by the insecticides was assessed by using RAPD-PCR analysis, and the results indicated Radiant as a genotoxic insecticide that caused large changes at the genomic-DNA level. On the other hand, Coragen exhibited the lowest mutability effect on insect DNA.
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