日本脑炎病毒SA14-14-2包膜蛋白的核苷酸序列

S. Hong, W. Yoo, R. Putnak, K. Eckels, H. Rho, Soo‐Ok Kim
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引用次数: 5

摘要

在原代犬肾细胞(PDK)中产生的减毒乙型脑炎病毒SA14-14-2适应于Vero细胞。为了深入了解SA14-14-2(Vero)毒株生物学特性的分子基础,测定了具有主要中和表位的包膜(E)基因编码的1500个核苷酸序列,并与另外两个乙脑减毒毒株SA14-14-2(PHK)和SA14-14-2(PDK)的序列进行了比较。3株菌株的c端(a.a 280 ~ 500)氨基酸序列相同,而n端(a.a 1 ~ 279)氨基酸序列不同。3株弱毒株的n端突变分布基本相同,提示n端序列可能与病毒-宿主细胞特异性有关。然而,发现已知负责衰减的Lys和Val(分别为a.a. 138和176)在SA14-14-2中仍然保守(Vero)。动物实验表明,SA14-14-2(Vero)在小鼠体内具有与亲本SA14-14-2(PDK)菌株相似的衰减表型。
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Nucleotide Sequence of Envelope Protein of Japanese Encephalitis Virus SA14-14-2 Adapted to Vero Cells
Live attenuated Japanese encephalitis (JE) virus SA14-14-2 produced in primary dog kidney cells (PDK) was adapted to Vero cells. In an effort to gain insight into the molecular basis of the biological characteristics of the SA14-14-2(Vero) strain, the 1500 nucleotide sequence encoding the envelope (E) gene which possesses major neutralizing epitopes was determined and compared with the sequences of two other attenuated JE virus strains, SA14-14-2(PHK) and SA14-14-2(PDK). The amino acid sequence of the C-terminal region (a.a. 280-500) was found to be identical for all three strains, while the N-terminal region (a.a. 1-279) shows sequence variation. The distribution of mutations in the N-terminal region was nearly the same among the three attenuated strains, suggesting that the N-terminal sequences might be related with virus-host cell specificity. However, it was found that Lys and Val (a.a. 138 and 176, respectively), known to be responsible for attenuation, are still conserved in SA14-14-2(Vero). Animal testing showed that SA14-14-2(Vero) has an attenuation phenotype similar to that of the parent s SA14-14-2(PDK) strain in mice.
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