应用聚合酶链反应检测伊朗骆驼流产病例中的伊文氏锥虫

Pub Date : 2020-03-01 DOI:10.3906/vet-1908-76

M. Nazem, Maryam Karimidehkordi, M. F. Moghadam

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引用次数: 3

摘要

伊文氏锥虫是一种血液寄生虫原生动物，可在牛和骆驼等多种经济价值动物中引起锥虫病或生存。这种感染导致骆驼流产。本研究采用聚合酶链式反应PCR方法对伊朗骆驼流产胎儿中伊文氏锥虫的存在进行了检测。在本研究中，从伊朗东部的一个伊朗骆驼群收集了244例流产胎儿的皱胃内容物。结果显示，244例流产胎儿中有41例(16.8%)感染伊氏锥虫DNA。结果表明，伊朗骆驼群中流产的高比例是由伊瓦氏锥虫感染引起的。流产胎儿伊万氏锥虫DNA阳性在不同取样地点差异有统计学意义(P < 0.05)。

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Detection of Trypanosoma evansi in camel abortions (Camelus dromedarius) in Iran using polymerase chain reaction

Trypanosoma evansi is a blood parasite protozoan that causes trypanosomiasis or surra in a variety of economically valued animals such as cattle and camels. This infection causes abortion in camels. The present study was conducted to evaluate the presence of Trypanosoma evansi in aborted fetuses of Iranian camels Camelus dromedarius by polymerase chain reaction PCR method. In this study, 244 abomasal contents of aborted fetuses were collected from an Iranian camel herd in the east of Iran. The results showed that 41 of 244 16.8% aborted fetuses were infected with Trypanosoma evansi DNA. The results showed that a high percentage of abortions in Iranian camel herds were due to Trypanosoma evansi infection. There was a significant difference in positive Trypanosoma evansi DNA in aborted fetuses between sampling locations P < 0.05 .

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