{"title":"坦桑尼亚东北部潘加尼地区蚊虫班氏乌切里氏菌感染流行情况","authors":"Godlisten S. Materu","doi":"10.24248/EASCI.V1I1.16","DOIUrl":null,"url":null,"abstract":"Background:\n Wuchereria bancrofti is the most\n widely distributed of the 3 nematodes known to cause lymphatic\n filariasis, the other 2 being Brugia malayi and\n Brugia timori. Anopheles gambiae\n and Anopheles funestus are the main\n vectors. However, the relative contributions of mosquito vectors to\n disease burden and infectivity are becoming increasingly important in\n coastal East Africa, and this is particularly true in the urban and\n semiurban areas of Pangani District, Tanzania. Methods: Mosquitoes were\n sampled from 5 randomly selected villages of Pangani District, namely,\n Bweni, Madanga, Meka, Msaraza, and Pangani West. Sampling of mosquitoes\n was done using standard Centers for Disease Control light traps with\n incandescent light bulbs. The presence of W. bancrofti\n in mosquitoes was determined via polymerase chain reaction\n (PCR) assays using NV1 and NV2 primers, and PoolScreen 2 software was\n used to determine the estimated rate of W. bancrofti\n infection in mosquitoes. Results: A total of 951\n mosquitoes were collected, of which 99.36% were Culex\n quinquefasciatus, 0.32% were Anopheles\n gambiae, and 0.32% other Culex species.\n The estimated rate of W. bancrofti infection among\n these mosquitoes was 3.3%. Conclusion: This was the\n first study employing the use of PoolScreen PCR to detect W.\n bancrofti circulating in mosquito vectors in Pangani\n District, northeastern Tanzania. The presence of W. bancrofti\n infection suggests the possibility of infected humans in the\n area. The high abundance of Cx. quinquefasciatus\n calls for integrated mosquito control interventions to\n minimise the risk of W. bancrofti transmission to\n humans. Further research is required to gain an in-depth understanding\n of the W. bancrofti larval stages in mosquitoes,\n their drug sensitivity and susceptibility profiles, and their\n fecundity.","PeriodicalId":11398,"journal":{"name":"East Africa Science","volume":"16 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence of Wuchereria bancrofti Infection in\\n Mosquitoes from Pangani District, Northeastern Tanzania\",\"authors\":\"Godlisten S. Materu\",\"doi\":\"10.24248/EASCI.V1I1.16\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background:\\n Wuchereria bancrofti is the most\\n widely distributed of the 3 nematodes known to cause lymphatic\\n filariasis, the other 2 being Brugia malayi and\\n Brugia timori. Anopheles gambiae\\n and Anopheles funestus are the main\\n vectors. However, the relative contributions of mosquito vectors to\\n disease burden and infectivity are becoming increasingly important in\\n coastal East Africa, and this is particularly true in the urban and\\n semiurban areas of Pangani District, Tanzania. Methods: Mosquitoes were\\n sampled from 5 randomly selected villages of Pangani District, namely,\\n Bweni, Madanga, Meka, Msaraza, and Pangani West. Sampling of mosquitoes\\n was done using standard Centers for Disease Control light traps with\\n incandescent light bulbs. The presence of W. bancrofti\\n in mosquitoes was determined via polymerase chain reaction\\n (PCR) assays using NV1 and NV2 primers, and PoolScreen 2 software was\\n used to determine the estimated rate of W. bancrofti\\n infection in mosquitoes. Results: A total of 951\\n mosquitoes were collected, of which 99.36% were Culex\\n quinquefasciatus, 0.32% were Anopheles\\n gambiae, and 0.32% other Culex species.\\n The estimated rate of W. bancrofti infection among\\n these mosquitoes was 3.3%. Conclusion: This was the\\n first study employing the use of PoolScreen PCR to detect W.\\n bancrofti circulating in mosquito vectors in Pangani\\n District, northeastern Tanzania. The presence of W. bancrofti\\n infection suggests the possibility of infected humans in the\\n area. The high abundance of Cx. quinquefasciatus\\n calls for integrated mosquito control interventions to\\n minimise the risk of W. bancrofti transmission to\\n humans. Further research is required to gain an in-depth understanding\\n of the W. bancrofti larval stages in mosquitoes,\\n their drug sensitivity and susceptibility profiles, and their\\n fecundity.\",\"PeriodicalId\":11398,\"journal\":{\"name\":\"East Africa Science\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-03-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"East Africa Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.24248/EASCI.V1I1.16\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"East Africa Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24248/EASCI.V1I1.16","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
背景:在已知的3种引起淋巴丝虫病的线虫中,bancroffwuchereria是分布最广泛的,另外2种是Brugia malayi和Brugia timori。冈比亚按蚊和富氏按蚊是主要病媒。然而,在东非沿海地区,蚊子媒介对疾病负担和传染性的相对贡献正变得越来越重要,在坦桑尼亚潘加尼区的城市和半城市地区尤其如此。方法:在潘加尼区Bweni、Madanga、Meka、Msaraza和Pangani West 5个村随机取样。蚊子的取样是用标准的疾病控制中心白炽灯泡诱捕器完成的。采用NV1和NV2引物,采用聚合酶链反应(PCR)法检测蚊虫中是否存在班氏怀特氏菌,并利用PoolScreen 2软件估算蚊虫中班氏怀特氏菌的感染率。结果:共捕获蚊虫951只,其中致倦库蚊占99.36%,冈比亚按蚊占0.32%,其他库蚊占0.32%。蚊群中班克罗夫特氏瓦氏菌感染率为3.3%。结论:本研究首次采用PoolScreen PCR检测坦桑尼亚东北部潘加尼地区蚊媒传播的班氏瓦氏菌。班氏杆菌感染的存在表明该地区可能有受感染的人。碳的高丰度。致倦库蚊呼吁采取综合蚊虫控制干预措施,以尽量减少班氏瓦氏体向人类传播的风险。需要进一步的研究来深入了解班氏瓦氏菌在蚊子体内的幼虫阶段、它们的药物敏感性和敏感性以及它们的繁殖力。
Prevalence of Wuchereria bancrofti Infection in
Mosquitoes from Pangani District, Northeastern Tanzania
Background:
Wuchereria bancrofti is the most
widely distributed of the 3 nematodes known to cause lymphatic
filariasis, the other 2 being Brugia malayi and
Brugia timori. Anopheles gambiae
and Anopheles funestus are the main
vectors. However, the relative contributions of mosquito vectors to
disease burden and infectivity are becoming increasingly important in
coastal East Africa, and this is particularly true in the urban and
semiurban areas of Pangani District, Tanzania. Methods: Mosquitoes were
sampled from 5 randomly selected villages of Pangani District, namely,
Bweni, Madanga, Meka, Msaraza, and Pangani West. Sampling of mosquitoes
was done using standard Centers for Disease Control light traps with
incandescent light bulbs. The presence of W. bancrofti
in mosquitoes was determined via polymerase chain reaction
(PCR) assays using NV1 and NV2 primers, and PoolScreen 2 software was
used to determine the estimated rate of W. bancrofti
infection in mosquitoes. Results: A total of 951
mosquitoes were collected, of which 99.36% were Culex
quinquefasciatus, 0.32% were Anopheles
gambiae, and 0.32% other Culex species.
The estimated rate of W. bancrofti infection among
these mosquitoes was 3.3%. Conclusion: This was the
first study employing the use of PoolScreen PCR to detect W.
bancrofti circulating in mosquito vectors in Pangani
District, northeastern Tanzania. The presence of W. bancrofti
infection suggests the possibility of infected humans in the
area. The high abundance of Cx. quinquefasciatus
calls for integrated mosquito control interventions to
minimise the risk of W. bancrofti transmission to
humans. Further research is required to gain an in-depth understanding
of the W. bancrofti larval stages in mosquitoes,
their drug sensitivity and susceptibility profiles, and their
fecundity.