SQUAMOSA启动子结合蛋白like7在拟南芥铜稳态调控中的应用[j]

Hiroaki Yamasaki, M. Hayashi, Mitsue Fukazawa, Y. Kobayashi, T. Shikanai
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引用次数: 430

摘要

在拟南芥中,miR398的表达在铜缺乏的情况下被诱导,并参与编码铜/锌超氧化物歧化酶的mrna的降解。我们发现SPL7 (SQUAMOSA启动子结合蛋白样7)对miR398的这种反应至关重要。SPL7与铜反应调节因子1同源,铜反应调节因子是莱茵衣藻在铜缺乏时在质体青素和细胞色素c6之间进行转换所必需的转录因子。在体外实验中,SPL7直接与miR398启动子中的GTAC基序结合,这些基序对于体内铜缺乏的反应是必要的和充分的。SPL7也是多个microrna miR397、miR408和miR857的表达所必需的,这些microrna参与铜稳态以及编码几种铜转运蛋白和铜伴侣的基因,这表明SPL7在铜缺乏反应中起着核心作用。与这一观点一致的是,在低铜条件下,spl7植株的生长受到严重损害。
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SQUAMOSA Promoter Binding Protein–Like7 Is a Central Regulator for Copper Homeostasis in Arabidopsis[W]
Expression of miR398 is induced in response to copper deficiency and is involved in the degradation of mRNAs encoding copper/zinc superoxide dismutase in Arabidopsis thaliana. We found that SPL7 (for SQUAMOSA promoter binding protein–like7) is essential for this response of miR398. SPL7 is homologous to Copper response regulator1, the transcription factor that is required for switching between plastocyanin and cytochrome c6 in response to copper deficiency in Chlamydomonas reinhardtii. SPL7 bound directly to GTAC motifs in the miR398 promoter in vitro, and these motifs were essential and sufficient for the response to copper deficiency in vivo. SPL7 is also required for the expression of multiple microRNAs, miR397, miR408, and miR857, involved in copper homeostasis and of genes encoding several copper transporters and a copper chaperone, indicating its central role in response to copper deficiency. Consistent with this idea, the growth of spl7 plants was severely impaired under low-copper conditions.
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