用于疾病建模、药物筛选和再生治疗的人类多能干细胞衍生类器官培养策略

Zakiya Gania, Syarifah Tiara Noorintan, Ni Putu Diah Pradnya Septiari, Dhea Sandra Fitriany, Fuad Gandhi Torizal
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引用次数: 5

摘要

人类多能干细胞(hPSCs)已成为生成人体各种细胞类型的有力工具。最近,类器官技术已经成为一个从psc中产生生理相关的组织样结构的平台。与实际的人体器官相比,这种结构比用于移植、疾病建模和药物开发的传统单层培养系统更接近于三维微环境。然而,尽管具有优势,类器官培养系统仍然存在各种与培养方法相关的问题,这些问题已经成为试图获得与原始组织相似的生理特性的挑战。在这里,我们讨论了目前类器官培养方法的发展,包括目前可用的培养系统可能出现的问题,以及克服它们目前的局限性和提高它们在转化应用目的中的最佳利用率的可能方法。
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Strategies for Generating Human Pluripotent Stem Cell-Derived-Organoid Culture for Disease Modeling, Drug Screening, and Regenerative Therapy
Human pluripotent stem cells (hPSCs) have become a powerful tool to generate the various kinds of cell types comprising the human body. Recently, organoid technology has emerged as a platform to generate a physiologically relevant tissue-like structure from PSCs. Compared to an actual human organ, this structure more closely represents a three-dimensional microenvironment than the conventional monolayer culture system for transplantation, disease modeling, and drug development. Despite its advantages, however, the organoid culture system still has various problems related to culture methods, which have become a challenge for attempts to obtain similar physiological properties to their original tissue counterparts. Here, we discuss the current development of organoid culture methods, including the problems that may arise from the currently available culture systems, as well as a possible approach for overcoming their current limitations and improving their optimum utilization for translational application purposes.
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