{"title":"中草药中地塞米松含量测定的简易分析方法","authors":"Rimadani Pratiwi, Adira Rahmawaty, A. N. Hasanah","doi":"10.1155/2022/5141647","DOIUrl":null,"url":null,"abstract":"Chemical drugs have been added to some of the traditional herbal medicine to enhance the therapeutic effect. One of them is dexamethasone which is added in herbal pain relief. To determine the content of dexamethasone in herbal medicine, a quantitative analytical method was developed with the reaction of the formation of a dexamethasone-hydroxylamine complex with fluoranil which was measured by UV-Vis spectrophotometry. Under optimized conditions, the performance of this system to analyze dexamethasone exhibited a good analytical response. The correlation coefficient was (R2) of 0.9965 from a concentration of 1–40 g/mL, LOD, and LOQ of 0.21 μg/mL and 0.64 μg/mL, % recovery for concentrations of 80%,100%, and 120%, are 100.14% ± 0.58; 98.35% ± 1.19; and 99.00% ± 1.18, respectively; and %RSD is 0.75%. The sixteen herbal medicine samples were analyzed to confirm the application of this method. The result showed six samples confirmed containing dexamethasone with levels ranging from 7.44 to 21.61 μg/mL and confirmed with HPLC data. The UV-Vis spectrophotometry method is simple and effective for dexamethasone analysis in herbal medicine.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Simple Analytical Method on the Determination of Dexamethasone in Herbal Medicine\",\"authors\":\"Rimadani Pratiwi, Adira Rahmawaty, A. N. Hasanah\",\"doi\":\"10.1155/2022/5141647\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Chemical drugs have been added to some of the traditional herbal medicine to enhance the therapeutic effect. One of them is dexamethasone which is added in herbal pain relief. To determine the content of dexamethasone in herbal medicine, a quantitative analytical method was developed with the reaction of the formation of a dexamethasone-hydroxylamine complex with fluoranil which was measured by UV-Vis spectrophotometry. Under optimized conditions, the performance of this system to analyze dexamethasone exhibited a good analytical response. The correlation coefficient was (R2) of 0.9965 from a concentration of 1–40 g/mL, LOD, and LOQ of 0.21 μg/mL and 0.64 μg/mL, % recovery for concentrations of 80%,100%, and 120%, are 100.14% ± 0.58; 98.35% ± 1.19; and 99.00% ± 1.18, respectively; and %RSD is 0.75%. The sixteen herbal medicine samples were analyzed to confirm the application of this method. The result showed six samples confirmed containing dexamethasone with levels ranging from 7.44 to 21.61 μg/mL and confirmed with HPLC data. The UV-Vis spectrophotometry method is simple and effective for dexamethasone analysis in herbal medicine.\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2022-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1155/2022/5141647\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1155/2022/5141647","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Simple Analytical Method on the Determination of Dexamethasone in Herbal Medicine
Chemical drugs have been added to some of the traditional herbal medicine to enhance the therapeutic effect. One of them is dexamethasone which is added in herbal pain relief. To determine the content of dexamethasone in herbal medicine, a quantitative analytical method was developed with the reaction of the formation of a dexamethasone-hydroxylamine complex with fluoranil which was measured by UV-Vis spectrophotometry. Under optimized conditions, the performance of this system to analyze dexamethasone exhibited a good analytical response. The correlation coefficient was (R2) of 0.9965 from a concentration of 1–40 g/mL, LOD, and LOQ of 0.21 μg/mL and 0.64 μg/mL, % recovery for concentrations of 80%,100%, and 120%, are 100.14% ± 0.58; 98.35% ± 1.19; and 99.00% ± 1.18, respectively; and %RSD is 0.75%. The sixteen herbal medicine samples were analyzed to confirm the application of this method. The result showed six samples confirmed containing dexamethasone with levels ranging from 7.44 to 21.61 μg/mL and confirmed with HPLC data. The UV-Vis spectrophotometry method is simple and effective for dexamethasone analysis in herbal medicine.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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