用计算机模拟分析热漂后常见沙拉蔬菜中维生素C(抗坏血酸)降解的动力学模型

E. Awagu, E. Ekanem, A. M. Kolo, M. Adamu
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引用次数: 11

摘要

维生素C(抗坏血酸)是最重要和最受欢迎的维生素之一,大多数水果和蔬菜中都含有维生素C;维生素C的问题在于它在加工过程中容易降解。在本研究中,测定了生菜和卷心菜中维生素C的降解动力学,并考虑了在70 - 0℃的水下分别焯水5、10、15、20、25、30、35和40 min的加工处理。样品在15-20℃的温和温度下干燥,研磨以寻找粉尘,采用高压液相色谱法(HPLC)测定蔬菜沙拉样品中的AA,该方法采用等温洗脱法,紫外可见检测波长为245nm。采用积分律法计算了两种蔬菜沙拉样品在相同处理方法下的速率常数;还计算了半衰期。在相同的预处理条件下,生菜和白菜中抗坏血酸的降解符合第一种动力学模型,决定系数(r2值)分别为0.8981和0.9785。在相同的焯水条件下,生菜和白菜抗坏血酸降解速率常数分别为0.099 min-1和0.088 min-1。生菜和卷心菜的半衰期分别为420.0892和472.6004秒。在焯水预处理条件下,白菜的速率常数较低,半衰期较长,降解速度较慢,是最合适的蔬菜沙拉。生菜的一级预测Ln(C)为0.657964,大白菜的一级预测Ln(C)为1.330017,进一步证明在相同预处理条件下,焯水生菜的降解率高于大白菜。
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Kinetic Modeling of Vitamin C (Ascorbic Acid) Degradation in Blanched Commonly Consumed Salad Vegetables Using Computer Simulation Analysis
Vitamin C (ascorbic acid) is one of the most important and popular vitamins, and is contained in most fruits and vegetables; the problem with vitamin C is its easy degradation during processing. In this study, the degradation kinetics of vitamin C was determined in Lettuce and Cabbage, and the processing treatment considered was blanching at 70 0 C of water differently for 5, 10, 15, 20,25,30,35 and 40 minutes. Samples were dried in mild temperature (15–20 0 C) and ground to find dust and High Pressure liquid chromatographic (HPLC) was used for determination of the AA of vegetable salad samples which consisted of an isocratic elution procedure with UV-Visible detection at 245nm. The rate constants were calculated for both vegetable salad samples under the same processing method using the integrated law method; half-life was also calculated. Degradation of ascorbic acid in Lettuce and Cabbage under the same pretreatment procedure followed the firstorder kinetic model, as the coefficient of determination (R 2 -value) were 0.8981 and 0.9785 respectively. The rate constant of ascorbic acid degradation for Lettuce and Cabbage under the same blanching conditions were 0.099 min-1 and 0.088 min-1 respectively. The half-life of Lettuce and Cabbage were 420.0892 and 472.6004 seconds respectively. The most appropriate vegetable salad under the blanching pretreatment procedure is the cabbage because its rate constant depicted from the model equations was lower, and the half life longer, hence, slower rate of degradation. The first order forecast (Ln(C)), was 0.657964 for Lettuce and 1.330017 for Cabbage, which further authenticate that blanched Lettuce degradation was higher than Cabbage under the same pretreatment conditions.
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