水稻染色体谷蛋白基因原位杂交定位

Hiroaki Suzuki, Y. Futsuhara, F. Takaiwa, N. Kurata
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引用次数: 9

摘要

利用水稻谷朊蛋白cDNA克隆与有丝分裂染色体原位杂交的方法,确定了储存蛋白谷朊蛋白基因在染色体上的位置。杂交后,分析了21个细胞的3h标记谷蛋白基因在染色体上的位置。发现银粒主要集中在2号染色体(K2染色体)的短臂上,说明负责储存蛋白谷氨酸的基因位于这条染色体上。
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Localization of glutelin gene in rice chromosome by in situ hybridization
The location of storage protein glutelin gene on a chromosome was determined by in situ hybridization made between glutelin cDNA clone and mitotic chromosomes in rice. After hybridization, the location of 3H-labelled glutelin gene was analyzed on chromosomes of twenty one cells. It was found that silver grains were mainly concentrated on the short arm of chromosome 2 (K2 chromosome), demonstrating that the gene responsible for storage protein glutelin was located on this chromosome.
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