T. Limongi, A. Giugni, H. Tan, Ebtihaj M Bukhari, B. Torre, M. Allione, M. Marini, L. Tirinato, G. Das, M. Moretti, A. Falqui, E. Fabrizio
{"title":"不同孔隙度聚己内酯支架的制备、压汞孔法表征及体外生物相容性定性分析","authors":"T. Limongi, A. Giugni, H. Tan, Ebtihaj M Bukhari, B. Torre, M. Allione, M. Marini, L. Tirinato, G. Das, M. Moretti, A. Falqui, E. Fabrizio","doi":"10.4172/2157-7552.1000159","DOIUrl":null,"url":null,"abstract":"In order to develop surfaces with improved cell culture biocompatibility, we optimized a solvent-casting and particulate-leaching fabrication technique to create porous three-dimensional polycaprolactone scaffolds. These biocompatible porous surfaces were realized by means of NaCl particles as porogen; salt leaching by immersion in distilled water created porosity and pore interconnectivity in the material. Scanning electron microscopy and mercury intrusion porosimetry were used for the measurement of porosity, pore size distribution, permeability and compressibility. To evaluate scaffold biocompatibility, fibroblasts were cultured on the porous surfaces and confocal immunofluorescence characterization indicated that they were effective for in vitro cell culture and practical tissue engineering applications.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"1 1","pages":"1-6"},"PeriodicalIF":0.0000,"publicationDate":"2015-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Fabrication, Mercury Intrusion Porosimetry Characterization and In Vitro Qualitative Analysis of Biocompatibility of Various Porosities Polycaprolactone Scaffolds\",\"authors\":\"T. Limongi, A. Giugni, H. Tan, Ebtihaj M Bukhari, B. Torre, M. Allione, M. Marini, L. Tirinato, G. Das, M. Moretti, A. Falqui, E. Fabrizio\",\"doi\":\"10.4172/2157-7552.1000159\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In order to develop surfaces with improved cell culture biocompatibility, we optimized a solvent-casting and particulate-leaching fabrication technique to create porous three-dimensional polycaprolactone scaffolds. These biocompatible porous surfaces were realized by means of NaCl particles as porogen; salt leaching by immersion in distilled water created porosity and pore interconnectivity in the material. Scanning electron microscopy and mercury intrusion porosimetry were used for the measurement of porosity, pore size distribution, permeability and compressibility. To evaluate scaffold biocompatibility, fibroblasts were cultured on the porous surfaces and confocal immunofluorescence characterization indicated that they were effective for in vitro cell culture and practical tissue engineering applications.\",\"PeriodicalId\":17539,\"journal\":{\"name\":\"Journal of Tissue Science and Engineering\",\"volume\":\"1 1\",\"pages\":\"1-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-12-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Tissue Science and Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/2157-7552.1000159\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Tissue Science and Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2157-7552.1000159","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Fabrication, Mercury Intrusion Porosimetry Characterization and In Vitro Qualitative Analysis of Biocompatibility of Various Porosities Polycaprolactone Scaffolds
In order to develop surfaces with improved cell culture biocompatibility, we optimized a solvent-casting and particulate-leaching fabrication technique to create porous three-dimensional polycaprolactone scaffolds. These biocompatible porous surfaces were realized by means of NaCl particles as porogen; salt leaching by immersion in distilled water created porosity and pore interconnectivity in the material. Scanning electron microscopy and mercury intrusion porosimetry were used for the measurement of porosity, pore size distribution, permeability and compressibility. To evaluate scaffold biocompatibility, fibroblasts were cultured on the porous surfaces and confocal immunofluorescence characterization indicated that they were effective for in vitro cell culture and practical tissue engineering applications.