A REVIEW ON INSTRUMENTATION AND VALIDATION METHOD OF UV-VISIBLE SPECTROSCOPY AND HPLC FOR THE ANALYSIS OF DRUGS

The purpose of the present analysis was to identify the application of HPLC and UV/Vis spectroscopy. UV-VIS Spectroscopy is the term used to test the various types of solvents and substances in an analysis. In particular, small-scale enterprises typically prefer spectroscopy, as the cost of equipment is lower and maintenance issues are limited. The analysis approach is based on the calculation of the absorption of monochromatic light in the near ultraviolet direction of a spectrum by colourless compounds (200 400nm). The pharmaceutical analysis requires the necessary procedure for the determination of the "identity, intensity, consistency and purity of such compounds. Analysis of raw materials and intermediates during the drug manufacturing process is also included. The dissociation constant is well known to be the most critical parameter in the production and optimization of a new compound for successful development of formulations. Information on the system of simultaneous equation, difference spectrophotometry, high-performance liquid chromatography (HPLC) or fluid visible ultraviolet spectrophotometry was measured. High performance liquid chromatography (HPLC) is an effective analytical method for drug product evaluation. Methods of HPLC should be able to isolate, detect and quantify the different drugs and drug related degradants that can shape, detect and quantify any drugs and drug related impurities that can be added during synthesis. Validation is the process of determining a method's performance features and limitations and defining the factors that which alter these characteristics and to what extent.