DETERMINATION OF THIAMAZOLE IN TABLET FORMULATION BY USING REVERSED PHASE LIQUID CHROMATOGRAPHIC METHOD

In this study, the amount of thiamazole (methimazole) in pharmaceutical tablet formulation was determined by using reversed phase liquid chromatography method (RPLC). Chromatographic separation was carried out by using YMC Triart C18 (150 mm × 4.6 mm, 3μm, YMC, USA) column. 5% (v/v) acetonitrile-water binary mixture at pH 9.5 was used as a mobile phase. Metronidazole was chosen as an internal standard. Flow rate was 0.8 ml/min and column temperature was 25 °C in chromatographic separation. The studied wavelengths for thiamazole and metronidazole are 260 and 340 nm, respectively. This proposed method was suitably validated with respect to accuracy, precision, linearity, the limit of detection (LOD) and limit of quantification (LOQ). The calibration graph of thiamazole was linear from 4 ppm to 14 ppm. The recovery of the 5 mg thiamazole containing commercial tablet (Thyromazol) was 100.059%. The proposed RPLC method was successfully applied to the determination of thiamazole in commercial tablet formulation.